Mycobacterium phage UPIE
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Detailed Information for Phage UPIE
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByShelby Paulson
Year Found2009
Location FoundCorvallis, OR USA
Finding InstitutionOregon State University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Unknown
Isolation TemperatureNot entered
GPS Coordinates44.565454 N, 123.275549 W Map
Discovery NotesDamp, dark soil next to plants and a pond next to the Oregon State library. 55°F
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedMar 17, 2011
Sequencing FacilityJoint Genomics Institute
Shotgun Sequencing MethodSanger Sequencing
Genome length (bp)73784
Character of genome ends3' Sticky Overhang
Overhang Length10 bases
GC Content58.8%
Fasta file available?Yes: Download fasta file
Characterization
ClusterL
SubclusterL1
Cluster Life CycleTemperate
Other Cluster Members
Annotating InstitutionUnknown or unassigned
Annotation StatusIn GenBank
Plaque Notes1-2mm; clear; incubated at 37 ºC usually for 20-24 hours
MorphotypeSiphoviridae
Number of Genes122
Number of tRNAs9
Number of tmRNAs0
Has been Phamerated?Yes
Gene List

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UPIE_1

UPIE_2
(terminase small subunit)

UPIE_3

UPIE_4
(terminase large subunit)

UPIE_5
(portal protein)

UPIE_6

UPIE_7
(scaffolding protein)

UPIE_8
(major capsid protein)

UPIE_9
(head-to-tail adaptor)

UPIE_10
(head-to-tail stopper)

UPIE_11

UPIE_12
(tail terminator)

UPIE_13
(major tail protein)

UPIE_14
(tail assembly chaperone)

UPIE_15
(tail assembly chaperone)

UPIE_16
(tape measure protein)

UPIE_17
(minor tail protein)

UPIE_18
(minor tail protein)

UPIE_19

UPIE_20

UPIE_21

UPIE_22

UPIE_23

UPIE_24

UPIE_25
(lysin A)

UPIE_26
(lysin B)

UPIE_27

UPIE_28

UPIE_29

UPIE_30
(NrdH-like glutaredoxin)

UPIE_31

UPIE_32
(nucleotidyl transferase)

UPIE_33
(Lsr2-like DNA bridging protein)

UPIE_34

UPIE_35

UPIE_36
(tyrosine integrase)

UPIE_37

UPIE_38

UPIE_39

UPIE_40

UPIE_41

UPIE_42

UPIE_43

UPIE_44

UPIE_45

UPIE_46

UPIE_47

UPIE_48

UPIE_49

UPIE_50

UPIE_51

UPIE_52

UPIE_53
(Ro-like RNA binding protein)

UPIE_54

UPIE_55

UPIE_56

UPIE_57

UPIE_58

UPIE_59

UPIE_60

UPIE_61
(ssDNA binding protein, ERF family)

UPIE_62

UPIE_63

UPIE_64

UPIE_65

UPIE_66

UPIE_67
(DnaB-like dsDNA helicase)

UPIE_68
(WhiB family transcription factor)

UPIE_69

UPIE_70

UPIE_71
(phosphoribosyl pyrophosphate transferase)

UPIE_72
(nicotinate ribosyltransferase)

UPIE_73

UPIE_74
(polynucleotide kinase)

UPIE_75

UPIE_76

UPIE_77
(WhiB family transcription factor)

UPIE_78
(WhiB family transcription factor)

UPIE_79

UPIE_80

UPIE_81

UPIE_82

UPIE_83

UPIE_84

UPIE_85
(RNA ligase)

UPIE_86

UPIE_87

UPIE_88

UPIE_89

UPIE_90
(HNH endonuclease)

UPIE_91

UPIE_92

UPIE_93
(DnaQ-like DNA polymerase III subunit)

UPIE_94

UPIE_95

UPIE_96
(endonuclease VII)

UPIE_97

UPIE_98

UPIE_99

UPIE_100

UPIE_101

UPIE_102 — tRNA
(codon recognized: UUG)

UPIE_103 — tRNA
(codon recognized: ACG)

UPIE_104

UPIE_105 — tRNA
(codon recognized: AAG)

UPIE_106 — tRNA
(codon recognized: UAC)

UPIE_107 — tRNA
(codon recognized: UGG)

UPIE_108 — tRNA
(codon recognized: CUA)

UPIE_109 — tRNA
(codon recognized: CAC)

UPIE_110

UPIE_111

UPIE_112 — tRNA
(codon recognized: UGC)

UPIE_113

UPIE_114 — tRNA
(codon recognized: AAA)

UPIE_115

UPIE_116

UPIE_117

UPIE_118

UPIE_119

UPIE_120

UPIE_121

UPIE_122

UPIE_123

UPIE_124

UPIE_125

UPIE_126

UPIE_127

UPIE_128
(AAA-ATPase)

UPIE_129

UPIE_130

UPIE_131

Publication Info
Uploaded to GenBank?Yes
GenBank AccessionJF704113
Refseq NumberNone yet
Published in PaperPope, Bowman, Russell, et al 2015, eLIFE: Whole genome comparison of a large collection of mycobacteriophages reveals a continuum of phage genetic diversity
Archiving Info
Archiving status Archived
SEA Designator 2009ORSUupieSNP
SEA Lysate Titer 2.1e11
Pitt Freezer Box# 3
Pitt Freezer Box Grid# C6
Available Files
Plaque PictureDownload
Virtual Digest PictureDownload
EM PictureDownload