| Detailed Information for Phage Underpass |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Alexa Rogers and Emma Wenzel |
| Year Found | 2024 |
| Location Found | Philadelphia, PA United States |
| Finding Institution | Saint Joseph's University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 37°C |
| GPS Coordinates | 39.99393 N, 75.24045 W Map |
| Discovery Notes | Soil collected at the SJU underpass construction site. The hole the soil came from was very deep, as it was dug with an excavator. The soil was very dry and dusty. A concrete underpass now lies over the site where we collected the soil.
Capsid diameter = 48.3 nm; Tail length = 188.6 nm (n = 6) |
| Naming Notes | Since the soil sample that our phage came from was found where the new Saint Joe's underpass was being built, we named it after that. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 10, 2025 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina NextSeq 1000 |
| Read Type | Single-end reads |
| Read Length | 100 bp |
| Approximate Shotgun Coverage | 1809 |
| Genome length (bp) | 71053 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | TCGATCAGCC |
| GC Content | 58.8% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | L |
| Subcluster | L2 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | Saint Joseph's University |
| Annotation Status | In GenBank |
| Plaque Notes | Our phage is lytic and approximately 2 mm in diameter. |
| Morphotype | Siphoviridae |
| Number of Genes | 122 |
| Number of tRNAs | 12 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | PV940982 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
213 |
| Pitt Freezer Box Grid# |
A7 |
| Available Files |
| Plaque Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
