| Detailed Information for Phage AmericanBeauty |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Andrew Jackson |
| Year Found | 2022 |
| Location Found | Whitesville, KY United States |
| Finding Institution | Western Kentucky University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 25°C |
| GPS Coordinates | 37.682141 N, 86.881305 W Map |
| Discovery Notes | Bacteriophage was pulled from a lawn at the coordinates stated. The soil was dry, and I dug about 8-10 mm. |
| Naming Notes | The name is one of my favorite albums of all time. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Apr 7, 2023 |
| Sequencing Facility | NC State Genomic Sciences Laboratory |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 2068 |
| Genome length (bp) | 75250 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 9 bases |
| Overhang Sequence | CGCTTGTCA |
| GC Content | 62.9% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | E |
| Subcluster | -- |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | Western Kentucky University |
| Annotation Status | In GenBank |
| Plaque Notes | 4 mm turbid plaques appeared after the second serial dilution. |
| Morphotype | Siphoviridae |
| Number of Genes | 147 |
| Number of tRNAs | 2 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | PQ559664 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
7.9x10^7 |
| Pitt Freezer Box# |
157 |
| Pitt Freezer Box Grid# |
F1 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
