Mycobacterium phage ChotaBhai
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Detailed Information for Phage ChotaBhai
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByAnas Gondal
Year Found2017
Location FoundBowling Green, KY USA
Finding InstitutionWestern Kentucky University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature30°C
GPS Coordinates36.98694 N, 86.45416 W Map
Discovery NotesSoil sample obtained outside of 1509 College Heights Blvd. on August 28th 2017. Humidity was 92% and pressure was 30 inHg. Winds were 4 mph, temperature was 70 degrees Fahrenheit. Sample was obtained at 10:02 P.M.
Naming NotesThe name means "Little Brother" in Urdu. The phages name can be paired with the name of the phage BaraBhai, which means big brother in Urdu
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedApr 13, 2018
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage197
Genome length (bp)76145
Character of genome ends3' Sticky Overhang
Overhang Length9 bases
Overhang SequenceCGCTTGTCA
GC Content62.9%
Fasta file available?Yes: Download fasta file
Characterization
ClusterE
Subcluster--
Cluster Life CycleTemperate
Other Cluster Members
Annotating InstitutionWestern Kentucky University
Annotation StatusIn GenBank
Plaque NotesPlaques were approximately 1 mm in width and lytic. Plaques were circular in shape and had a smooth border
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List

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ChotaBhai_1

ChotaBhai_2

ChotaBhai_3

ChotaBhai_4

ChotaBhai_5

ChotaBhai_6
(terminase small subunit)

ChotaBhai_7

ChotaBhai_8
(terminase large subunit)

ChotaBhai_9

ChotaBhai_10
(portal protein)

ChotaBhai_11
(capsid maturation protease)

ChotaBhai_12
(major capsid protein)

ChotaBhai_13

ChotaBhai_14
(head-to-tail adaptor)

ChotaBhai_15
(head-to-tail stopper)

ChotaBhai_16

ChotaBhai_17
(tail terminator)

ChotaBhai_18
(major tail protein)

ChotaBhai_19
(capsid decoration protein)

ChotaBhai_20
(tail assembly chaperone)

ChotaBhai_21
(tail assembly chaperone)

ChotaBhai_22
(tape measure protein)

ChotaBhai_23
(minor tail protein)

ChotaBhai_24
(minor tail protein)

ChotaBhai_25
(minor tail protein)

ChotaBhai_26
(minor tail protein)

ChotaBhai_27
(minor tail protein)

ChotaBhai_28
(minor tail protein)

ChotaBhai_29

ChotaBhai_30

ChotaBhai_31
(membrane protein)

ChotaBhai_32
(lysin A)

ChotaBhai_33
(holin)

ChotaBhai_34

ChotaBhai_35
(lysin B)

ChotaBhai_36
(membrane protein)

ChotaBhai_37
(NrdH-like glutaredoxin)

ChotaBhai_38

ChotaBhai_39
(Lsr2-like DNA bridging protein)

ChotaBhai_40

ChotaBhai_41

ChotaBhai_42

ChotaBhai_43

ChotaBhai_44

ChotaBhai_45

ChotaBhai_46

ChotaBhai_47

ChotaBhai_48

ChotaBhai_49

ChotaBhai_50

ChotaBhai_51
(membrane protein)

ChotaBhai_52
(integrase)

ChotaBhai_53

ChotaBhai_54
(membrane protein)

ChotaBhai_55
(immunity repressor)

ChotaBhai_56

ChotaBhai_57
(membrane protein)

ChotaBhai_58
(pentapeptide repeat protein)

ChotaBhai_59

ChotaBhai_60

ChotaBhai_61

ChotaBhai_62

ChotaBhai_63

ChotaBhai_64

ChotaBhai_65

ChotaBhai_66

ChotaBhai_67
(exonuclease)

ChotaBhai_68

ChotaBhai_69
(ERF family ssDNA binding protein)

ChotaBhai_70

ChotaBhai_71

ChotaBhai_72

ChotaBhai_73

ChotaBhai_74

ChotaBhai_75

ChotaBhai_76
(DprA-like DNA processing chain A)

ChotaBhai_77
(HNH endonuclease)

ChotaBhai_78

ChotaBhai_79

ChotaBhai_80

ChotaBhai_81
(DnaB-like dsDNA helicase)

ChotaBhai_82

ChotaBhai_83
(WhiB family transcription factor)

ChotaBhai_84

ChotaBhai_85

ChotaBhai_86
(holliday junction resolvase)

ChotaBhai_87
(membrane protein)

ChotaBhai_88
(polynucleotide kinase)

ChotaBhai_89

ChotaBhai_90
(membrane protein)

ChotaBhai_91
(RNA ligase)

ChotaBhai_92
(helix-turn-helix DNA binding domain protein)

ChotaBhai_93
(WhiB family transcription factor)

ChotaBhai_94

ChotaBhai_95

ChotaBhai_96

ChotaBhai_97

ChotaBhai_98

ChotaBhai_99
(methyltransferase)

ChotaBhai_100
(ssDNA binding protein)

ChotaBhai_101
(ClpP-like protease)

ChotaBhai_102

ChotaBhai_103

ChotaBhai_104

ChotaBhai_105
(excise)

ChotaBhai_106
(HNH endonuclease)

ChotaBhai_109

ChotaBhai_110

ChotaBhai_111
(hydrolase)

ChotaBhai_112

ChotaBhai_113

ChotaBhai_114
(DNA polymerase III subunit)

ChotaBhai_115

ChotaBhai_116

ChotaBhai_117

ChotaBhai_118

ChotaBhai_119
(RecA-like DNA recombinase)

ChotaBhai_120

ChotaBhai_121

ChotaBhai_122

ChotaBhai_123

ChotaBhai_124

ChotaBhai_125

ChotaBhai_126

ChotaBhai_127

ChotaBhai_128

ChotaBhai_129

ChotaBhai_130

ChotaBhai_131

ChotaBhai_132

ChotaBhai_133

ChotaBhai_134

ChotaBhai_135

ChotaBhai_136

ChotaBhai_137

ChotaBhai_138

ChotaBhai_139

ChotaBhai_140

ChotaBhai_141

ChotaBhai_142

ChotaBhai_143

ChotaBhai_144

ChotaBhai_145

ChotaBhai_146

ChotaBhai_147

ChotaBhai_148

ChotaBhai_149

ChotaBhai_150

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMK801726
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 51
Pitt Freezer Box Grid# G3
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload