The Actinobacteriophage Database

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Detailed Information for Phage Tuco
Discovery Information
Isolation Host	Mycobacterium smegmatis mc²155
Found By	Eddy Santos
Year Found	2013
Location Found	Nyack, NY USA
Finding Institution	Alliance University (formerly Nyack College)
Program	Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?	Yes
Isolation Temperature	37°C
GPS Coordinates	41.081909 N, 73.927521 W Map
Discovery Notes	Hee Jae Ko found this soil sample by a tree by Simpson Hall at Nyack College. This soil sample had two types of phages named Tuco and Beesknees in it. Eddy Santos worked on Tuco while Beesknees was worked on by Greg Mastrangelo.
Naming Notes	Tuco was a character in the TV show series Breaking Bad
Sequencing Information
Sequencing Complete?	Yes
Date Sequencing Completed	Jan 3, 2014
Sequencing Facility	NC State Genomic Sciences Laboratory
Shotgun Sequencing Method	Illumina Sequencing
Approximate Shotgun Coverage	4765
Genome length (bp)	76944
Character of genome ends	3' Sticky Overhang
Overhang Length	9 bases
Overhang Sequence	CGCTTGTCA
GC Content	63.0%
Fasta file available?	Yes: Download fasta file
Characterization
Cluster	E
Subcluster	--
Cluster Life Cycle	Temperate
Other Cluster Members	Click to View 244 ABCat Adnama Amao AmericanBeauty Argent26 Asriel BaboJay BadStone Balomoji Barbarian Bask21 Bench BigBubba BilboSwaggins Bruin Buck BugsBunny Cactus ChosenOne ChotaBhai Cjw1 Command613 Contagion Cookies CrystalP Czyszczon1 Daikon DoctorDiddles DrDrey Dumbo Dusk Easy2Say Elite2014 Elph10 Emmina Eureka Filch FireRed Flypotenuse Gage Gator Gemini Glexan Goku GoldenSpark Goldilocks GooberAzure HanKaySha Harella Henry Highbury Holt Hoonter Hopey HufflyPuff Icee IHOP Inca JeTaime Kanye Kimchi Kostya Lilac Lilizi Lilpickle MadamMonkfish Manda Marshmallow Maxxinista Mindy Miniwave Misfit MISSy Moldemort Mosby MPhalcon Murica Murphy Myrale Nala NelitzaMV Nimrod NoSleep OrionPax Palpatine Paperbeatsrock Pat3 Petra64142 Phaja Pharsalus PhatBacter Phaux Phrux Policronamos Porky Pumpkin Quallification Rakim Rimmer RiverMonster Saints25 Sassay ShamWow ShereKhan Simpliphy SirDuracell SophKB Sotrice96 Stank Stark StellaBean StolenFromERC Tarkin TBrady12 TeardropMSU Teaspoon Terminus Thresher Tomaszewski Toto Traaww1 Tuco Ukulele Wiggin Willez Xandras xkcd YassJohnny Youngblood
Annotating Institution	Unknown or unassigned
Annotation Status	In GenBank
Plaque Notes	A lot of small and clear plaques.
Morphotype	Siphoviridae
Has been Phamerated?	Yes
Gene List	Click to View Tuco_1 Tuco_2 Tuco_3 Tuco_4 Tuco_5 Tuco_6 (terminase small subunit) Tuco_7 Tuco_8 Tuco_9 (terminase large subunit) Tuco_10 Tuco_11 (portal protein) Tuco_12 (capsid maturation protease) Tuco_13 (major capsid protein) Tuco_14 Tuco_15 (head-to-tail adaptor) Tuco_16 (head-to-tail stopper) Tuco_17 (head-to-tail connector protein) Tuco_18 (tail terminator) Tuco_19 (major tail protein) Tuco_20 (tail assembly chaperone) Tuco_21 (tail assembly chaperone) Tuco_22 (HNH endonuclease) Tuco_23 (tape measure protein) Tuco_24 (minor tail protein) Tuco_25 (minor tail protein) Tuco_26 (minor tail protein) Tuco_27 (minor tail protein) Tuco_28 (minor tail protein) Tuco_29 Tuco_30 Tuco_31 (HTH DNA binding protein) Tuco_32 (membrane domain protein) Tuco_33 (lysin A) Tuco_34 (holin) Tuco_35 Tuco_36 (lysin B) Tuco_37 (membrane domain protein) Tuco_38 (glutaredoxin) Tuco_39 (HNH endonuclease) Tuco_40 Tuco_41 (DNA bridging protein) Tuco_42 Tuco_43 Tuco_44 Tuco_45 Tuco_46 Tuco_47 Tuco_48 Tuco_49 Tuco_50 Tuco_51 Tuco_52 (membrane domain protein) Tuco_53 (tyrosine integrase) Tuco_54 Tuco_55 (membrane domain protein) Tuco_56 (immunity repressor) Tuco_57 Tuco_58 (membrane domain protein) Tuco_59 (pentapeptide repeat protein) Tuco_60 Tuco_61 Tuco_62 Tuco_63 Tuco_64 Tuco_65 Tuco_66 Tuco_67 Tuco_68 Tuco_69 Tuco_70 (ssDNA binding protein, ERF family) Tuco_71 Tuco_72 Tuco_73 Tuco_74 Tuco_75 (membrane domain protein) Tuco_76 Tuco_77 (DNA processing chain A) Tuco_78 (HNH endonuclease) Tuco_79 Tuco_80 Tuco_81 Tuco_82 (dsDNA helicase) Tuco_83 Tuco_84 (WhiB family transcription factor) Tuco_85 Tuco_86 Tuco_87 (holliday junction resolvase) Tuco_88 Tuco_89 (polynucleotide kinase) Tuco_90 Tuco_91 Tuco_92 (RNA ligase) Tuco_93 (HTH DNA binding protein) Tuco_94 (WhiB family transcription factor) Tuco_95 Tuco_96 Tuco_97 Tuco_98 Tuco_99 Tuco_100 (DNA methylase) Tuco_101 (ssDNA binding protein) Tuco_102 (ClpP-like protease) Tuco_103 Tuco_104 Tuco_105 Tuco_106 (HTH DNA binding protein) Tuco_107 (HNH endonuclease) Tuco_110 Tuco_111 Tuco_112 (hydrolase) Tuco_113 Tuco_114 Tuco_115 (DnaQ-like DNA polymerase III subunit) Tuco_116 Tuco_117 Tuco_118 Tuco_119 Tuco_120 (RecA-like DNA recombinase) Tuco_121 Tuco_122 Tuco_123 Tuco_124 Tuco_125 Tuco_126 Tuco_127 Tuco_128 Tuco_129 Tuco_130 Tuco_131 Tuco_132 Tuco_133 (helix-turn-helix DNA binding domain protein) Tuco_134 Tuco_135 Tuco_136 Tuco_137 Tuco_138 Tuco_139 Tuco_140 Tuco_141 Tuco_142 Tuco_143 Tuco_144 Tuco_145 Tuco_146 Tuco_147 Tuco_148 Tuco_149 Tuco_150 (HNH endonuclease)
Submitted Annotation in DNA Master Format	Download
Submitted Minimal DNA Master File	Download
Author List	Download
Annotation Cover Sheet	Download
Publication Info
Uploaded to GenBank?	Yes
GenBank Accession	KX817173
Refseq Number	None yet
Published in Paper	Pope, Bowman, Russell, et al 2015, eLIFE: Whole genome comparison of a large collection of mycobacteriophages reveals a continuum of phage genetic diversity
Archiving Info
Archiving status	Archived
SEA Lysate Titer	2.4x10^10
Pitt Freezer Box#	1
Pitt Freezer Box Grid#	C12
Available Files
EM Picture	Download
Final DNAMaster File	Download
GenBank File for Phamerator	Download

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