The Actinobacteriophage Database

Mycobacterium phage Youngblood

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Detailed Information for Phage Youngblood
Discovery Information
Isolation Host	Mycobacterium smegmatis mc²155
Found By	Team Bad Assay
Year Found	2014
Location Found	Los Angeles, CA USA
Finding Institution	University of California, Los Angeles
Program	Unavailable
From enriched soil sample?	Yes
Isolation Temperature	35°C
GPS Coordinates	34.075 N, 118.451 W Map
Discovery Notes	Phage isolation performed in Spring 2014 and genome annotation performed in Fall 2014 by Team Bad Assay: Arash Amighi, Chaya Patel, Yvonne Lam, Steven Youngblood. Annotation quality checked by Edric Yoon and Lei Yeh. DNA preparation performed by Dr. Krisanavane Reddi (UCLA). Assembly performed by Dr. Jordan Moberg Parker (UCLA). Sample description: Dry, dark brown, grainy soil collected at a 6cm depth by Yvonne Lam on April 4, 2014. Area is urban and flat. The location is near trees and foot traffic.
Naming Notes	Named after team member Steven Youngblood.
Sequencing Information
Sequencing Complete?	Yes
Date Sequencing Completed	Jan 23, 2015
Sequencing Facility	The UCLA Genotyping and Sequencing Core
Shotgun Sequencing Method	454 Pyrosequencing
Approximate Shotgun Coverage	38
Genome length (bp)	75896
Character of genome ends	3' Sticky Overhang
Overhang Length	9 bases
Overhang Sequence	CGCTTGTCA
GC Content	62.9%
Fasta file available?	Yes: Download fasta file
Characterization
Cluster	E
Subcluster	--
Cluster Life Cycle	Temperate
Lysogeny Notes	Evidence for lysogeny: bullseye plaque morphology, and genome contains integrase gene.
Other Cluster Members	Click to View 244 ABCat Adnama Amao AmericanBeauty Argent26 Asriel BaboJay BadStone Balomoji Barbarian Bask21 Bench BigBubba BilboSwaggins Bruin Buck Cactus ChosenOne ChotaBhai Cjw1 Command613 Contagion Cookies CrystalP Czyszczon1 Daikon DoctorDiddles DrDrey Dumbo Dusk Easy2Say Elite2014 Elph10 Emmina Eureka Filch FireRed Flypotenuse Gage Gator Gemini Glexan Goku GoldenSpark Goldilocks GooberAzure HanKaySha Harella Henry Highbury Holt Hoonter Hopey HufflyPuff Icee IHOP Inca JeTaime Kanye Kimchi Kostya Lilac Lilizi Lilpickle MadamMonkfish Manda Marshmallow Maxxinista Mindy Miniwave Misfit MISSy Moldemort Mosby MPhalcon Murica Murphy Myrale Nala NelitzaMV Nimrod NoSleep OrionPax Paperbeatsrock Pat3 Petra64142 Phaja Pharsalus PhatBacter Phaux Phrux Porky Pumpkin Quallification Rakim Rimmer RiverMonster Saints25 Sassay ShamWow ShereKhan Simpliphy SirDuracell SophKB Sotrice96 Stank Stark StellaBean StolenFromERC Tarkin TBrady12 TeardropMSU Teaspoon Terminus Thresher Tomaszewski Toto Traaww1 Tuco Ukulele Wiggin Willez Xandras xkcd YassJohnny Youngblood
Annotating Institution	Unknown or unassigned
Annotation Status	In GenBank
Plaque Notes	The plaque morphology had a bullseye or turbid morphology depending on the dilution. The plaques were circular ranging from 0.2 to 0.5 cm with the average size of 3.37 mm and standard deviation of 0.43 mm.
Morphotype	Siphoviridae
Number of Genes	148
Number of tRNAs	2
Has been Phamerated?	Yes
Gene List	Click to View Youngblood_1 Youngblood_2 Youngblood_3 Youngblood_4 Youngblood_5 (terminase small subunit) Youngblood_6 Youngblood_7 Youngblood_8 (terminase large subunit) Youngblood_9 Youngblood_10 (portal protein) Youngblood_11 (capsid maturation protease) Youngblood_12 (major capsid protein) Youngblood_13 Youngblood_14 (head-to-tail adaptor) Youngblood_15 (head-to-tail stopper) Youngblood_16 (head-to-tail connector protein) Youngblood_17 (tail terminator) Youngblood_18 (major tail protein) Youngblood_19 Youngblood_20 (tail assembly chaperone) Youngblood_21 (tail assembly chaperone) Youngblood_22 (tape measure protein) Youngblood_23 (minor tail protein) Youngblood_24 (minor tail protein) Youngblood_25 (minor tail protein) Youngblood_26 (minor tail protein) Youngblood_27 (D-ala-D-ala carboxypeptidase) Youngblood_28 (minor tail protein) Youngblood_29 Youngblood_30 (helix-turn-helix DNA binding domain protein) Youngblood_31 Youngblood_32 (lysin A) Youngblood_33 (holin) Youngblood_34 Youngblood_35 (lysin B) Youngblood_36 Youngblood_37 (glutaredoxin) Youngblood_38 (DNA bridging protein) Youngblood_39 Youngblood_40 Youngblood_41 Youngblood_42 Youngblood_43 Youngblood_44 Youngblood_45 Youngblood_46 Youngblood_47 Youngblood_48 Youngblood_49 Youngblood_50 Youngblood_51 Youngblood_52 Youngblood_53 (membrane domain protein) Youngblood_54 (tyrosine integrase) Youngblood_55 Youngblood_56 Youngblood_57 (immunity repressor) Youngblood_58 Youngblood_59 Youngblood_60 (pentapeptide repeat protein) Youngblood_61 Youngblood_62 Youngblood_63 Youngblood_64 Youngblood_65 Youngblood_66 Youngblood_67 Youngblood_68 Youngblood_69 Youngblood_70 Youngblood_71 Youngblood_72 (ERF family ssDNA binding protein) Youngblood_73 Youngblood_74 Youngblood_75 Youngblood_76 Youngblood_77 Youngblood_78 Youngblood_79 Youngblood_80 (HNH endonuclease) Youngblood_81 Youngblood_82 Youngblood_83 Youngblood_84 (dsDNA helicase) Youngblood_85 Youngblood_86 (WhiB family transcription factor) Youngblood_87 Youngblood_88 Youngblood_89 (holliday junction resolvase) Youngblood_90 Youngblood_91 (polynucleotide kinase) Youngblood_92 Youngblood_93 Youngblood_94 (RNA ligase) Youngblood_95 (helix-turn-helix DNA binding protein) Youngblood_96 (WhiB family transcription factor) Youngblood_97 Youngblood_98 Youngblood_99 Youngblood_100 Youngblood_101 Youngblood_102 (DNA methylase) Youngblood_103 (ssDNA binding protein) Youngblood_104 (ClpP-like protease) Youngblood_105 Youngblood_106 Youngblood_107 Youngblood_108 (helix-turn-helix DNA binding protein) Youngblood_109 (HNH endonuclease) Youngblood_110 Youngblood_113 Youngblood_114 Youngblood_115 Youngblood_116 Youngblood_117 (DnaQ-like DNA polymerase III subunit) Youngblood_118 Youngblood_119 (RecA-like DNA recombinase) Youngblood_120 Youngblood_121 Youngblood_122 Youngblood_123 Youngblood_124 Youngblood_125 Youngblood_126 Youngblood_127 Youngblood_128 Youngblood_129 Youngblood_130 Youngblood_131 Youngblood_132 (helix-turn-helix DNA binding domain protein) Youngblood_133 Youngblood_134 Youngblood_135 Youngblood_136 Youngblood_137 Youngblood_138 Youngblood_139 Youngblood_140 Youngblood_141 Youngblood_142 Youngblood_143 Youngblood_144 Youngblood_145 Youngblood_146 Youngblood_147 Youngblood_148 Youngblood_149 Youngblood_150 (HNH endonuclease)
Submitted Minimal DNA Master File	Download
Publication Info
Uploaded to GenBank?	Yes
GenBank Accession	MG099953
Refseq Number	None yet
Archiving Info
Archiving status	Archived
Pitt Freezer Box#	23
Pitt Freezer Box Grid#	E3
Available Files
Plaque Picture	Download
Restriction Digest Picture	Download
EM Picture	Download
GenBank File for Phamerator	Download

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