Detailed Information for Phage Contagion |
Discovery Information |
Isolation Host | Mycobacterium smegmatis mc²155 |
Found By | Paola Lockwood |
Year Found | 2011 |
Location Found | Westchester, CA USA |
Finding Institution | Loyola Marymount University |
Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
From enriched soil sample? | Yes |
Isolation Temperature | Not entered |
GPS Coordinates | 33.969875 N, 118.420014 W Map |
Discovery Notes | Sunny day approximately 69°F. Located on the campus of LMU by the sophomore housing, specifically the covered garden in front of McCarthy Hall. Soil sample was taken 2-3inches below the surface by a blossoming tree's roots. Other plants (flowers,grass) were in the near area. The dirt was moist. |
Sequencing Information |
Sequencing Complete? | Yes |
Date Sequencing Completed | Jan 12, 2012 |
Sequencing Facility | Virginia Commonwealth University Nucleic Acids Research Facilities |
Shotgun Sequencing Method | 454 Pyrosequencing |
Approximate Shotgun Coverage | 80 |
Genome length (bp) | 74533 |
Character of genome ends | 3' Sticky Overhang |
Overhang Length | 9 bases |
Overhang Sequence | CGCTTGTCA |
GC Content | 63.1% |
Fasta file available? | Yes: Download fasta file |
Characterization |
Cluster | E |
Subcluster | -- |
Cluster Life Cycle | Temperate |
Other Cluster Members |
|
Annotating Institution | Unknown or unassigned |
Annotation Status | In GenBank |
Plaque Notes | Lysogenic. Fuzzy concentric rings around a cock-eye lysed center. Approximately 0.1 cm in diameter after 24-48 hours. |
Morphotype | Siphoviridae |
Number of Genes | 140 |
Number of tRNAs | 2 |
Has been Phamerated? | Yes |
Gene List |
|
Publication Info |
Uploaded to GenBank? | Yes |
GenBank Accession | KF024732 |
Refseq Number | NC_022065 |
Published in Paper | Pope, Bowman, Russell, et al 2015, eLIFE: Whole genome comparison of a large collection of mycobacteriophages reveals a continuum of phage genetic diversity |
Archiving Info |
Archiving status |
Archived |
SEA Designator |
2011LOMAcontagionPL |
SEA Lysate Titer |
2.11e12 |
Date of SEA Lysate Titering |
Oct 24, 2011 |
Pitt Freezer Box# |
25 |
Pitt Freezer Box Grid# |
4 |
Available Files |
Plaque Picture | Download |
Restriction Digest Picture | Download |
EM Picture | Download |