Mycobacterium phage Bench
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Detailed Information for Phage Bench
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByZach Romero
Year Found2021
Location FoundBowling Green, KY United States
Finding InstitutionWestern Kentucky University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature30°C
GPS Coordinates36.986333 N, 86.454611 W Map
Discovery NotesLocation: in front of McLean Hall Sign on WKU Campus
Soil: Damp; included organic matter such as sticks and beads/rocks
Depth: 2 inches below the soil
Ambient Temperature: 27.7 degrees Celsius
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 21, 2022
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage1438
Genome length (bp)76092
Character of genome ends3' Sticky Overhang
Overhang Length9 bases
Overhang SequenceCGCTTGTCA
GC Content63.1%
Fasta file available?Yes: Download fasta file
Characterization
ClusterE
Subcluster--
Cluster Life CycleTemperate
Other Cluster Members
Annotating InstitutionWestern Kentucky University
Annotation StatusIn GenBank
Plaque NotesPlaques can be categorized as "bullseye." Larger plaques have significant halos around them, while smaller plaques are less distinct. The bullseye plaques indicate a lytic phage that has not fully lysed a cell, or is less efficient at during lysis. Most of the plaque shows clearness, while the outside of the cell indicates turbidity.
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List

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Bench_1

Bench_2

Bench_3

Bench_4

Bench_5

Bench_6
(terminase, small subunit)

Bench_7

Bench_8

Bench_9

Bench_10
(terminase, large subunit)

Bench_11

Bench_12
(portal protein)

Bench_13
(capsid maturation protease)

Bench_14
(major capsid protein)

Bench_15

Bench_16
(head-to-tail adaptor)

Bench_17
(head-to-tail stopper)

Bench_18

Bench_19
(tail terminator)

Bench_20
(major tail protein)

Bench_21
(capsid decoration protein)

Bench_22
(tail assembly chaperone)

Bench_23
(tail assembly chaperone)

Bench_24
(tape measure protein)

Bench_25
(minor tail protein)

Bench_26
(minor tail protein)

Bench_27
(minor tail protein)

Bench_28
(minor tail protein)

Bench_29
(minor tail protein, D-ala-D-ala carboxypeptidase)

Bench_30

Bench_31

Bench_32
(helix-turn-helix DNA binding domain)

Bench_33

Bench_34
(lysin A)

Bench_35
(holin)

Bench_36

Bench_37
(lysin B)

Bench_38

Bench_39
(NrdH-like glutaredoxin)

Bench_40

Bench_41
(Lsr2-like DNA bridging protein)

Bench_42

Bench_43

Bench_44

Bench_45

Bench_46

Bench_47

Bench_48

Bench_49

Bench_50

Bench_51

Bench_52

Bench_53

Bench_54
(tyrosine integrase)

Bench_55

Bench_56

Bench_57
(immunity repressor)

Bench_58

Bench_59

Bench_60
(pentapeptide repeat protein)

Bench_61

Bench_62

Bench_63

Bench_64

Bench_65

Bench_66

Bench_67

Bench_68
(memb)

Bench_69

Bench_70
(exonuclease)

Bench_71

Bench_72
(ssDNA binding protein, ERF family)

Bench_73

Bench_74

Bench_75

Bench_76

Bench_77

Bench_78

Bench_79

Bench_80
(HNH endonuclease)

Bench_81

Bench_82

Bench_83

Bench_84
(DnaB-like dsDNA helicase)

Bench_85

Bench_86
(WhiB family transcription factor)

Bench_87

Bench_88

Bench_89
(Holliday junction resolvase)

Bench_90

Bench_91
(polynucleotide kinase)

Bench_92

Bench_93

Bench_94
(RNA ligase)

Bench_95
(helix-turn-helix DNA binding domain)

Bench_96
(WhiB family transcription factor)

Bench_97

Bench_98

Bench_99

Bench_100

Bench_101

Bench_102
(DNA methyltransferase)

Bench_103
(ssDNA binding protein)

Bench_104
(ClpP-like protease)

Bench_105

Bench_106

Bench_107
(helix-turn-helix DNA binding domain)

Bench_108
(HNH endonuclease)

Bench_111

Bench_112

Bench_113
(hydrolase)

Bench_114

Bench_115
(DnaQ-like (DNA polymerase III subunit))

Bench_116

Bench_117
(RecA-like DNA recombinase)

Bench_118

Bench_119

Bench_120

Bench_121

Bench_122

Bench_123

Bench_124

Bench_125

Bench_126

Bench_127

Bench_128

Bench_129

Bench_130
(ParB-like nuclease domain)

Bench_131

Bench_132

Bench_133

Bench_134

Bench_135

Bench_136

Bench_137

Bench_138

Bench_139

Bench_140

Bench_141

Bench_142

Bench_143

Bench_144

Bench_145

Bench_146

Bench_147

Bench_148

Bench_149
(HNH endonuclease)

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionON456345
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Lysate Titer 2.24 x 10^10 pfu/ml
Pitt Freezer Box# 137
Pitt Freezer Box Grid# G3
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload