| Detailed Information for Phage Holt |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Morgan Holt |
| Year Found | 2016 |
| Location Found | Bowling Green, KY USA |
| Finding Institution | Western Kentucky University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 30°C |
| GPS Coordinates | 36.93262 N, 86.40674 W Map |
| Discovery Notes | This soil came from a compost pile in Bowling Green, Ky. The compost pile was rich in nutrients (composed of leaves and debris). The soil was dark in color and moist, but not muddy. The sample was collected on September 15, 2016. |
| Naming Notes | This phage was named after its founder, Morgan Holt. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Feb 6, 2023 |
| Sequencing Facility | NC State Genomic Sciences Laboratory |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 198 |
| Genome length (bp) | 75915 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 9 bases |
| Overhang Sequence | CGCTTGTCA |
| GC Content | 62.9% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | E |
| Subcluster | -- |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | Western Kentucky University |
| Annotation Status | In GenBank |
| Plaque Notes | The plaques were very easy to see because they were clear and not turbid. The plaques were also easy to pick due to their medium size and visibility. |
| Morphotype | Siphoviridae |
| Number of Genes | 150 |
| Number of tRNAs | 2 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | OR253902 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Designator |
2016WEKUholtMH |
| SEA Lysate Titer |
7.7 x 10^11 pfu/mL |
| Date of SEA Lysate Titering |
Nov 1, 2016 |
| Pitt Freezer Box# |
37 |
| Pitt Freezer Box Grid# |
C6 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
