| Detailed Information for Phage Willez |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Pham et al. |
| Year Found | 2011 |
| Location Found | Culver City, CA USA |
| Finding Institution | University of California, Los Angeles |
| Program | Unavailable |
| From enriched soil sample? | Yes |
| Isolation Temperature | 35°C |
| GPS Coordinates | 34.0 N, 118.0 W Map |
| Discovery Notes | Soil sample collected from potted plant in backyard of home in Culver City by Tiffany Pham.
Phage isolated and characterized by Team Awesome: Danny Fernandez, Tiffany Pham, Dennis Sambolin, and Steve Zhang. Instructor: Todd Lorenz. TA: Jovo Vijanderan. Laboratory Support: Krisanavane Reddi. Annoted by Debbie Jacobs-Sera, Edric Yoon and Lei Yeh. |
| Naming Notes | Previously names Phagebook (MSPB02). Renamed Willez in May 2013. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Aug 6, 2013 |
| Sequencing Facility | The UCLA Genotyping and Sequencing Core |
| Shotgun Sequencing Method | 454 Pyrosequencing |
| Approximate Shotgun Coverage | 157 |
| Genome length (bp) | 74576 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 9 bases |
| Overhang Sequence | CGCTTGTCA |
| GC Content | 62.9% |
| Sequencing Notes | 10% of reads assemble into a cluster A genome. |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | E |
| Subcluster | -- |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | Unknown or unassigned |
| Annotation Status | In GenBank |
| Plaque Notes | Turbid, 0.5 cm plaques |
| Morphotype | Siphoviridae |
| Number of Genes | 142 |
| Number of tRNAs | 2 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MF919540 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
3 |
| Pitt Freezer Box Grid# |
G2 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| Final DNAMaster File | Download |
| GenBank File for Phamerator | Download |
