Mycobacterium phage Misfit
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Detailed Information for Phage Misfit
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Former namesTerminator
Found ByBrownstead, Hilary; Gustafson, Eric; Sayeed, Aatif
Year Found2010
Location FoundCorona, CA USA
Finding InstitutionWashington University in St. Louis
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation TemperatureNot entered
GPS Coordinates33.84125 N, 117.549055 W Map
Discovery NotesCollected on 09/04/2010 at 4:40 pm.
Air temperature: 38ºC
Moist Soil, 4cm deep
An urban setting, 1 meter from a patch of grass. The sample location is adjacent to a wooden divider and artificial white stones. The sample was surrounded by tomato plants and 15 cm away from peppermint plants. The sample location is .6 meters from an apple tree. The sample area was in a vegetable garden of a single-family home (about 1.5 meters away from the foundation). The area is watered daily.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedNov 6, 2019
Sequencing FacilityThe McDonnell Genome Institute at Washington University
Shotgun Sequencing Method454 Pyrosequencing
Approximate Shotgun Coverage181
Sanger Finishing Reactions14
Genome length (bp)76169
Character of genome ends3' Sticky Overhang
Overhang Length9 bases
Overhang SequenceCGCTTGTCA
GC Content63.1%
Fasta file available?Yes: Download fasta file
Characterization
ClusterE
Subcluster--
Cluster Life CycleTemperate
Other Cluster Members
Annotating InstitutionWashington University in St. Louis
Annotation StatusBeing Annotated (Expected completion by 5/15/2020)
Plaque NotesThe plaques range in size from 0.5mm to 2.0mm in diameter. Plaques are turbid and clear.
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List
Publication Info
Uploaded to GenBank?No
GenBank AccessionNone yet
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Designator WUSL
Date of SEA Lysate Titering Nov 2, 2010
Pitt Freezer Box# 20
Pitt Freezer Box Grid# 27
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload