Mycobacterium phage Misfit
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Detailed Information for Phage Misfit
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Former namesTerminator
Found ByBrownstead, Hilary; Gustafson, Eric; Sayeed, Aatif
Year Found2010
Location FoundCorona, CA USA
Finding InstitutionWashington University in St. Louis
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation TemperatureNot entered
GPS Coordinates33.84125 N, 117.549055 W Map
Discovery NotesCollected on 09/04/2010 at 4:40 pm.
Air temperature: 38ºC
Moist Soil, 4cm deep
An urban setting, 1 meter from a patch of grass. The sample location is adjacent to a wooden divider and artificial white stones. The sample was surrounded by tomato plants and 15 cm away from peppermint plants. The sample location is .6 meters from an apple tree. The sample area was in a vegetable garden of a single-family home (about 1.5 meters away from the foundation). The area is watered daily.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedNov 6, 2019
Sequencing FacilityThe McDonnell Genome Institute at Washington University
Shotgun Sequencing Method454 Pyrosequencing
Approximate Shotgun Coverage181
Sanger Finishing Reactions14
Genome length (bp)76169
Character of genome ends3' Sticky Overhang
Overhang Length9 bases
Overhang SequenceCGCTTGTCA
GC Content63.1%
Fasta file available?Yes: Download fasta file
Characterization
ClusterE
Subcluster--
Cluster Life CycleTemperate
Other Cluster Members
Annotating InstitutionWashington University in St. Louis
Annotation StatusIn GenBank
Plaque NotesThe plaques range in size from 0.5mm to 2.0mm in diameter. Plaques are turbid and clear.
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List

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Misfit_1

Misfit_2

Misfit_3

Misfit_4

Misfit_5
(terminase small subunit)

Misfit_6

Misfit_7

Misfit_8
(terminase large subunit)

Misfit_9
(RNA binding protein)

Misfit_10
(portal protein)

Misfit_11
(capsid maturation protease)

Misfit_12
(major capsid protein)

Misfit_13

Misfit_14
(head-to-tail adaptor)

Misfit_15
(head-to-tail stopper)

Misfit_16

Misfit_17
(tail terminator)

Misfit_18
(major tail protein)

Misfit_19
(head fiber protein)

Misfit_20
(tail assembly chaperone)

Misfit_21
(tail assembly chaperone)

Misfit_22
(tape measure protein)

Misfit_23
(minor tail protein)

Misfit_24
(minor tail protein)

Misfit_25
(minor tail protein)

Misfit_26
(minor tail protein)

Misfit_27
(minor tail protein)

Misfit_28

Misfit_29

Misfit_30
(helix-turn-helix DNA binding domain protein)

Misfit_31

Misfit_32
(lysin A)

Misfit_33
(holin)

Misfit_34

Misfit_35
(lysin B)

Misfit_36

Misfit_37
(NrdH-like glutaredoxin)

Misfit_38
(Lsr2-like DNA bridging protein)

Misfit_39

Misfit_40

Misfit_41

Misfit_42

Misfit_43

Misfit_44

Misfit_45

Misfit_46

Misfit_47

Misfit_48

Misfit_49

Misfit_50

Misfit_51

Misfit_52

Misfit_53

Misfit_54
(tyrosine integrase)

Misfit_55

Misfit_56

Misfit_57
(immunity repressor)

Misfit_58

Misfit_59

Misfit_60
(pentapeptide repeat protein)

Misfit_61

Misfit_62

Misfit_63

Misfit_64

Misfit_65

Misfit_66

Misfit_67

Misfit_68
(Cas4 family exonuclease)

Misfit_69

Misfit_70
(ERF family ssDNA binding protein)

Misfit_71

Misfit_72

Misfit_73

Misfit_74

Misfit_75

Misfit_76

Misfit_77

Misfit_78
(HNH endonuclease)

Misfit_79

Misfit_80

Misfit_81

Misfit_82
(DnaB-like dsDNA helicase)

Misfit_83

Misfit_84
(WhiB family transcription factor)

Misfit_85

Misfit_86

Misfit_87
(holliday junction resolvase)

Misfit_88

Misfit_89
(polynucleotide kinase)

Misfit_90

Misfit_91

Misfit_92
(RNA ligase)

Misfit_93
(helix-turn-helix DNA binding domain protein)

Misfit_94
(WhiB family transcription factor)

Misfit_95

Misfit_96

Misfit_97

Misfit_98

Misfit_99

Misfit_100
(methyltransferase)

Misfit_101
(ssDNA binding protein)

Misfit_102
(ClpP-like protease)

Misfit_103

Misfit_104

Misfit_105

Misfit_106
(excise)

Misfit_107
(HNH endonuclease)

Misfit_110

Misfit_111

Misfit_112
(nucleoside deoxyribosyltransferase)

Misfit_113

Misfit_114
(DNA polymerase III subunit)

Misfit_115

Misfit_116
(RecA-like DNA recombinase)

Misfit_117

Misfit_118

Misfit_119

Misfit_120

Misfit_121

Misfit_122

Misfit_123

Misfit_124

Misfit_125

Misfit_126

Misfit_127

Misfit_128
(helix-turn-helix DNA binding domain protein)

Misfit_129

Misfit_130

Misfit_131

Misfit_132

Misfit_133

Misfit_134

Misfit_135

Misfit_136

Misfit_137

Misfit_138

Misfit_139

Misfit_140

Misfit_141

Misfit_142

Misfit_143

Misfit_144

Misfit_145

Misfit_146

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMT522005
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Designator WUSL
Date of SEA Lysate Titering Nov 2, 2010
Pitt Freezer Box# 20
Pitt Freezer Box Grid# 27
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload