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Mycobacterium phage Tarkin
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Detailed Information for Phage Tarkin
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Former namesDion
Found ByEthan Dionne
Year Found2018
Location FoundProvidence, RI USA
Finding InstitutionProvidence College
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature37°C
GPS Coordinates41.843 N, 71.438 W Map
Discovery NotesA soil sample was collected outside of the Albertus Magnus Science Building, Providence College, Providence, RI on September 5, 2018 at 4pm. The sample was slightly moist, clay-like top soil taken from a mostly shady grassy area during partly cloudy conditions, 27.8°C with 68% humidity.
Naming NotesThe name "Tarkin" comes from the Star Wars villain Grand Moff Tarkin, who was in charge of the Death Star's operations. I likened Tarkin's role in operating a weapon of planetary destruction to phage, which "fire" their DNA into a host, eventually resulting in the host's destruction.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedAug 31, 2020
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage911
Genome length (bp)75998
Character of genome ends3' Sticky Overhang
Overhang Length9 bases
Overhang SequenceCGCTTGTCA
GC Content63.0%
Fasta file available?Yes: Download fasta file
Characterization
ClusterE
Subcluster--
Cluster Life CycleTemperate
Other Cluster Members
Annotating InstitutionProvidence College
Annotation StatusIn GenBank
Plaque NotesPlaques are relatively small, clear in color, and distinct against the bacterial lawn.
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List

Click to View

Tarkin_1

Tarkin_2

Tarkin_3

Tarkin_4

Tarkin_5

Tarkin_6
(terminase, small subunit)

Tarkin_7

Tarkin_8

Tarkin_9

Tarkin_10
(terminase, large subunit)

Tarkin_11

Tarkin_12
(portal protein)

Tarkin_13
(capsid maturation protease)

Tarkin_14
(major capsid protein)

Tarkin_15

Tarkin_16
(head-to-tail adaptor)

Tarkin_17
(head-to-tail stopper)

Tarkin_18

Tarkin_19
(tail terminator)

Tarkin_20
(major tail protein)

Tarkin_21

Tarkin_22
(tail assembly chaperone)

Tarkin_23
(tail assembly chaperone)

Tarkin_24
(tape measure protein)

Tarkin_25
(minor tail protein)

Tarkin_26
(minor tail protein)

Tarkin_27
(minor tail protein)

Tarkin_28
(minor tail protein)

Tarkin_29
(minor tail protein, D-ala-D-ala carboxypeptidase)

Tarkin_30

Tarkin_31

Tarkin_32
(helix-turn-helix DNA binding protein)

Tarkin_33

Tarkin_34
(lysin A)

Tarkin_35
(holin)

Tarkin_36

Tarkin_37
(lysin B)

Tarkin_38

Tarkin_39
(NrdH-like glutaredoxin)

Tarkin_40
(Lsr2-like DNA bridging protein)

Tarkin_41

Tarkin_42

Tarkin_43

Tarkin_44

Tarkin_45

Tarkin_46

Tarkin_47

Tarkin_48

Tarkin_49

Tarkin_50

Tarkin_51
(tyrosine integrase)

Tarkin_52

Tarkin_53

Tarkin_54
(immunity repressor)

Tarkin_55

Tarkin_56

Tarkin_57
(pentapeptide repeat protein)

Tarkin_58

Tarkin_59

Tarkin_60

Tarkin_61

Tarkin_62

Tarkin_63

Tarkin_64

Tarkin_65

Tarkin_66

Tarkin_67

Tarkin_68
(ssDNA binding protein, ERF family)

Tarkin_69

Tarkin_70

Tarkin_71

Tarkin_72

Tarkin_73

Tarkin_74

Tarkin_75
(DprA-like DNA processing chain A)

Tarkin_76
(HNH endonuclease)

Tarkin_77

Tarkin_78

Tarkin_79

Tarkin_80
(DNA-B like dsDNA helicase)

Tarkin_81

Tarkin_82
(WhiB family transcription factor)

Tarkin_83

Tarkin_84

Tarkin_85
(holliday junction resolvase)

Tarkin_86

Tarkin_87
(polynucleotide kinase)

Tarkin_88

Tarkin_89

Tarkin_90
(RNA ligase)

Tarkin_91
(helix-turn-helix DNA binding protein)

Tarkin_92
(WhiB family transcription factor)

Tarkin_93

Tarkin_94

Tarkin_95

Tarkin_96

Tarkin_97

Tarkin_98
(DNA methylase)

Tarkin_99
(ssDNA binding protein)

Tarkin_100
(Clp-like protease)

Tarkin_101

Tarkin_102

Tarkin_103

Tarkin_104

Tarkin_105
(HNH endonuclease)

Tarkin_108

Tarkin_109

Tarkin_110
(deoxycytidylate deaminase)

Tarkin_111

Tarkin_112
(DnaQ-like (DNA polymerase III subunit))

Tarkin_113

Tarkin_114
(RecA-like DNA recombinase)

Tarkin_115

Tarkin_116

Tarkin_117

Tarkin_118

Tarkin_119

Tarkin_120

Tarkin_121

Tarkin_122

Tarkin_123

Tarkin_124

Tarkin_125

Tarkin_126
(helix-turn-helix DNA binding protein)

Tarkin_127

Tarkin_128

Tarkin_129

Tarkin_130

Tarkin_131

Tarkin_132

Tarkin_133

Tarkin_134

Tarkin_135

Tarkin_136

Tarkin_137

Tarkin_138

Tarkin_139

Tarkin_140

Tarkin_141

Tarkin_142

Tarkin_143

Tarkin_144
(HNH endonuclease)

Submitted Annotation in DNA Master FormatDownload
Submitted Minimal DNA Master FileDownload
Author ListDownload
Annotation Cover SheetDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMZ681505
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Lysate Titer 2.3*10^10
Pitt Freezer Box# 89
Pitt Freezer Box Grid# E2
Available Files
Plaque PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload