Mycobacterium phage Cactus
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Detailed Information for Phage Cactus
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByJalen Smith
Year Found2011
Location FoundAtlanta, GA USA
Finding InstitutionMorehouse College
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation TemperatureNot entered
GPS Coordinates33.748326 N, 84.414507 W Map
Discovery Notessaturated soil, 1 inch depth, 86 Fahrenheit, The soil was found in a shady area.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedFeb 3, 2012
Genome length (bp)75603
Character of genome ends3' Sticky Overhang
Overhang Length9 bases
Overhang SequenceCGCTTGTCA
GC Content63.0%
Fasta file available?Yes: Download fasta file
Characterization
ClusterE
Subcluster--
Cluster Life CycleTemperate
Other Cluster Members
Annotating InstitutionPurdue University
Annotation StatusIn GenBank
Plaque Notes1942 micrometers when measured. The plaque is clear.
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List

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Cactus_1

Cactus_2

Cactus_3

Cactus_4

Cactus_5

Cactus_6
(terminase small subunit)

Cactus_7

Cactus_8

Cactus_9

Cactus_10
(terminase large subunit)

Cactus_11

Cactus_12
(portal protein)

Cactus_13
(capsid maturation protease)

Cactus_14
(major capsid protein)

Cactus_15

Cactus_16
(head-to-tail adaptor)

Cactus_17
(head-to-tail stopper)

Cactus_18

Cactus_19
(tail terminator)

Cactus_20
(major tail protein)

Cactus_21
(tail assembly chaperone)

Cactus_22
(tail assembly chaperone)

Cactus_23
(tape measure protein)

Cactus_24
(minor tail protein)

Cactus_25
(minor tail protein)

Cactus_26
(minor tail protein)

Cactus_27
(minor tail protein)

Cactus_28
(minor tail protein)

Cactus_29
(minor tail protein)

Cactus_30

Cactus_31
(helix-turn-helix DNA binding domain protein)

Cactus_32

Cactus_33
(lysin A)

Cactus_34
(holin)

Cactus_35

Cactus_36
(lysin B)

Cactus_37
(NrdH-like glutaredoxin)

Cactus_38
(NrdH-like glutaredoxin)

Cactus_39
(Lsr2-like DNA bridging protein)

Cactus_40

Cactus_41

Cactus_42

Cactus_43

Cactus_44

Cactus_45

Cactus_46

Cactus_47

Cactus_48

Cactus_49

Cactus_50

Cactus_51

Cactus_52

Cactus_53

Cactus_54
(tyrosine integrase)

Cactus_55

Cactus_56

Cactus_57
(immunity repressor)

Cactus_58

Cactus_59

Cactus_60
(pentapeptide repeat protein)

Cactus_61

Cactus_62

Cactus_63

Cactus_64

Cactus_65

Cactus_66

Cactus_67

Cactus_68

Cactus_69

Cactus_70
(exonuclease)

Cactus_71

Cactus_72
(ERF family ssDNA binding protein)

Cactus_73

Cactus_74

Cactus_75

Cactus_76

Cactus_77

Cactus_78

Cactus_79

Cactus_80
(HNH endonuclease)

Cactus_81

Cactus_82

Cactus_83

Cactus_84
(DnaB-like dsDNA helicase)

Cactus_85

Cactus_86
(WhiB family transcription factor)

Cactus_87

Cactus_88

Cactus_89
(holliday junction resolvase)

Cactus_90

Cactus_91
(polynucleotide kinase)

Cactus_92

Cactus_93

Cactus_94
(RNA ligase)

Cactus_95

Cactus_96
(WhiB family transcription factor)

Cactus_97

Cactus_98

Cactus_99

Cactus_100

Cactus_101

Cactus_102

Cactus_103

Cactus_104
(ssDNA binding protein)

Cactus_105
(ClpP-like protease)

Cactus_106

Cactus_107

Cactus_108

Cactus_109

Cactus_110
(HNH endonuclease)

Cactus_113

Cactus_114

Cactus_115
(nucleoside deoxyribosyltransferase)

Cactus_116

Cactus_117

Cactus_118
(dnaQ-like DNA polymerase III subunit)

Cactus_119

Cactus_120

Cactus_121

Cactus_122

Cactus_123
(RecA-like DNA recombinase)

Cactus_124

Cactus_125

Cactus_126

Cactus_127

Cactus_128

Cactus_129

Cactus_130

Cactus_131

Cactus_132

Cactus_133

Cactus_134

Cactus_135

Cactus_136

Cactus_137

Cactus_138

Cactus_139

Cactus_140

Cactus_141

Cactus_142

Cactus_143

Cactus_144

Cactus_145

Cactus_146

Cactus_147

Cactus_148

Cactus_149

Cactus_150

Cactus_151

Cactus_152

Cactus_153

Cactus_154

Submitted Annotation in DNA Master FormatDownload
Submitted Minimal DNA Master FileDownload
Author ListDownload
Annotation Cover SheetDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMT723943
Refseq NumberNone yet
Published in PaperPope, Bowman, Russell, et al 2015, eLIFE: Whole genome comparison of a large collection of mycobacteriophages reveals a continuum of phage genetic diversity
Archiving Info
Archiving status Archived
SEA Designator 2011MOREcactusJTS
SEA Lysate Titer 5.52e10
Date of SEA Lysate Titering Nov 30, 2011
Pitt Freezer Box# 26
Pitt Freezer Box Grid# 58
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload