Mycobacterium phage Goldilocks

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Detailed Information for Phage Goldilocks
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByEvelyn Lewis and Grahm Skowronski
Year Found2015
Location FoundNorfolk, VA USA
Finding InstitutionOld Dominion University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?No
Isolation TemperatureNot entered
GPS CoordinatesUnavailable
Naming NotesIt took a while to get the conditions just right for this phage to be properly purified thus affectionately being named Goldilocks
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 5, 2016
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage366
Genome length (bp)75728
Character of genome ends3' Sticky Overhang
Overhang Length9 bases
Overhang SequenceCGCTTGTCA
GC Content62.9%
Fasta file available?Available upon entering GPS coordinates
Characterization
ClusterE
Subcluster--
Cluster Life CycleTemperate
Other Cluster Members
Annotating InstitutionUnknown or unassigned
Annotation StatusIn GenBank
Plaque NotesGoldilocks displays multiple plaque morphological types; a 0.5mm temperate plaque and a 2.5mm temperate plaque. A total of 10 purifications were done before doing an EM that showed homogeneous viruses.
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List

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Submitted Annotation in DNA Master FormatDownload
Submitted Minimal DNA Master FileDownload
Author ListDownload
Annotation Cover SheetDownload
Publication Info
Uploaded to GenBank?No
GenBank AccessionNone yet
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 22
Pitt Freezer Box Grid# E1
Available Files
Final DNAMaster FileDownload
GenBank File for PhameratorDownload