Mycobacterium phage Quallification
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Detailed Information for Phage Quallification
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByAllyson Qualley, Makenzie Cutillo
Year Found2020
Location FoundNahant, MA USA
Finding InstitutionMerrimack College
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?No
Isolation Temperature21°C
GPS Coordinates42.426597 N, 70.923828 W Map
Naming NotesAllyson and Makenzie both succeeded in isolating phage using the direct isolation method. Allyson's phage was lost during the amplification step, probably due to having to stay in the fridge too long during a COVID campus shutdown. Makenzie succeeded in amplifying hers and isolating its DNA. So, Makenzie contributed the phage, Allyson contributed the name, a play on her last name, Qualley.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 20, 2021
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage828
Genome length (bp)75908
Character of genome ends3' Sticky Overhang
Overhang Length9 bases
Overhang SequenceCGCTTGTCA
GC Content63.0%
Fasta file available?Yes: Download fasta file
Characterization
ClusterE
Subcluster--
Cluster Life CycleTemperate
Other Cluster Members
Annotating InstitutionMerrimack College
Annotation StatusIn GenBank
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List

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Quallification_1

Quallification_2

Quallification_3

Quallification_4

Quallification_5

Quallification_6
(terminase, small subunit)

Quallification_7

Quallification_8

Quallification_9

Quallification_10
(terminase, large subunit)

Quallification_11
(RNA binding protein)

Quallification_12
(portal protein)

Quallification_13
(capsid maturation protease)

Quallification_14
(major capsid protein)

Quallification_15

Quallification_16
(head-to-tail adaptor)

Quallification_17
(head-to-tail stopper)

Quallification_18

Quallification_19
(tail terminator)

Quallification_20
(major tail protein)

Quallification_21
(tail assembly chaperone)

Quallification_22
(tail assembly chaperone)

Quallification_23
(tape measure protein)

Quallification_24
(minor tail protein)

Quallification_25
(minor tail protein)

Quallification_26
(minor tail protein)

Quallification_27
(minor tail protein)

Quallification_28
(minor tail protein, D-ala-D-ala carboxypeptidase)

Quallification_29

Quallification_30

Quallification_31
(helix-turn-helix DNA binding domain)

Quallification_32

Quallification_33
(lysin A)

Quallification_34
(holin)

Quallification_35

Quallification_36
(lysin B)

Quallification_37

Quallification_38
(NrdH-like glutaredoxin)

Quallification_39
(Lsr2-like DNA bridging protein)

Quallification_40

Quallification_41

Quallification_42

Quallification_43

Quallification_44

Quallification_45

Quallification_46

Quallification_47

Quallification_48

Quallification_49

Quallification_50

Quallification_51

Quallification_52

Quallification_53
(tyrosine integrase)

Quallification_54

Quallification_55

Quallification_56
(immunity repressor)

Quallification_57

Quallification_58

Quallification_59
(pentapeptide repeat protein)

Quallification_60

Quallification_61

Quallification_62

Quallification_63

Quallification_64

Quallification_65

Quallification_66

Quallification_67

Quallification_68
(Cas4 family exonuclease)

Quallification_69

Quallification_70
(ssDNA binding protein, ERF family)

Quallification_71

Quallification_72

Quallification_73

Quallification_74

Quallification_75

Quallification_76

Quallification_77
(DprA-like DNA processing chain A)

Quallification_78
(HNH endonuclease)

Quallification_79

Quallification_80

Quallification_81

Quallification_82
(DnaB-like dsDNA helicase)

Quallification_83

Quallification_84
(WhiB family transcription factor)

Quallification_85

Quallification_86

Quallification_87
(Holliday junction resolvase)

Quallification_88

Quallification_89
(polynucleotide kinase)

Quallification_90

Quallification_91

Quallification_92
(RNA ligase)

Quallification_93
(helix-turn-helix DNA binding domain)

Quallification_94
(WhiB family transcription factor)

Quallification_95

Quallification_96

Quallification_97

Quallification_98

Quallification_99

Quallification_100
(DNA methylase)

Quallification_101
(ssDNA binding protein)

Quallification_102
(ClpP-like protease)

Quallification_103

Quallification_104

Quallification_105

Quallification_106
(helix-turn-helix DNA binding domain)

Quallification_107
(HNH endonuclease)

Quallification_110

Quallification_111

Quallification_112
(deoxycytidylate deaminase)

Quallification_113

Quallification_114
(DnaQ-like (DNA polymerase III subunit))

Quallification_115

Quallification_116
(RecA-like DNA recombinase)

Quallification_117

Quallification_118

Quallification_119

Quallification_120

Quallification_121

Quallification_122

Quallification_123

Quallification_124

Quallification_125

Quallification_126

Quallification_127

Quallification_128
(helix-turn-helix DNA binding domain)

Quallification_129

Quallification_130

Quallification_131

Quallification_132

Quallification_133

Quallification_134

Quallification_135

Quallification_136

Quallification_137

Quallification_138

Quallification_139

Quallification_140

Quallification_141

Quallification_142

Quallification_143

Quallification_144

Quallification_145

Quallification_146
(HNH endonuclease)

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionON970600
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 124
Pitt Freezer Box Grid# G4
Available Files
Plaque PictureDownload
GenBank File for PhameratorDownload