Mycobacterium phage Gemini
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Detailed Information for Phage Gemini
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByEric Westerman
Year Found2010
Location Foundrural, WV USA
Finding InstitutionWestern Kentucky University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation TemperatureNot entered
GPS Coordinates38.770916 N, 81.31445 W Map
Discovery NotesThe soil type was fertile garden soil.
I dug 8.89 centimeters into the soil.
The temperature was 68 degrees Fahrenheit.
On top of a large hill in West Virginia, near Spenser, WV.

I found the sample in a vegetable garden which cantained peppers and tomatoes. Being in the area of a former pasture field and a current residential area, the ground was relatively flat, and the soil must have been tread on often during the gardening process. The garden was surrounded by a fence, but other than that, the closest structure was a driveway, which was approximately 25ft away. The soil was relatively moist, and it should yield some interesting phage do to its fertility.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedMar 1, 2016
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage2875
Genome length (bp)75649
Character of genome ends3' Sticky Overhang
Overhang Length9 bases
Overhang SequenceCGCTTGTCA
GC Content62.9%
Fasta file available?Yes: Download fasta file
Characterization
ClusterE
Subcluster--
Cluster Life CycleTemperate
Other Cluster Members
Annotation StatusIn GenBank
Plaque Notes0.4cm clear plaques with turbid haloes, and also 0.1cm plaques that look clear at first glance, but on closer inspection also have thinner haloes. Of particular note is the unwillingness of plaques to overtake each other, usually forming around instead of over other plaques. Best seen in relationship between big halo and small plaques. Forms half moon shape. 24 hour incubation period in 37°C conditions.
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List

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Gemini_1

Gemini_2

Gemini_3

Gemini_4

Gemini_5

Gemini_6
(terminase small subunit)

Gemini_7
(membrane protein)

Gemini_8

Gemini_9

Gemini_10
(terminase large subunit)

Gemini_11

Gemini_12
(portal protein)

Gemini_13
(capsid maturation protease)

Gemini_14
(major capsid protein)

Gemini_15
(head-to-tail connector complex protein)

Gemini_16
(head-to-tail connector complex protein)

Gemini_17
(head-to-tail connector complex protein)

Gemini_18
(head-to-tail connector complex protein)

Gemini_19
(head-to-tail connector complex protein)

Gemini_20
(major tail protein)

Gemini_21

Gemini_22
(tail assembly chaperone)

Gemini_23
(tail assembly chaperone)

Gemini_24
(HNH endonuclease)

Gemini_25
(tape measure protein)

Gemini_26
(minor tail protein)

Gemini_27
(minor tail protein)

Gemini_28
(minor tail protein)

Gemini_29
(minor tail protein)

Gemini_30
(D-ala-D-ala carboxypeptidase)

Gemini_31
(minor tail protein)

Gemini_32

Gemini_33
(helix-turn-helix DNA-binding domain protein)

Gemini_34

Gemini_35
(lysin A)

Gemini_36
(holin)

Gemini_37

Gemini_38
(lysin B)

Gemini_39
(membrane protein)

Gemini_40
(NrdH-like glutaredoxin)

Gemini_41
(Lsr2-like DNA bridging protein)

Gemini_42

Gemini_43

Gemini_44

Gemini_45

Gemini_46

Gemini_47

Gemini_48

Gemini_49

Gemini_50

Gemini_51

Gemini_52

Gemini_53

Gemini_54

Gemini_55
(membrane protein)

Gemini_56
(integrase)

Gemini_57

Gemini_58
(membrane protein)

Gemini_59
(immunity repressor)

Gemini_60

Gemini_61
(membrane protein)

Gemini_62
(pentapeptide repeat protein)

Gemini_63

Gemini_64

Gemini_65

Gemini_66

Gemini_67

Gemini_68

Gemini_69

Gemini_70

Gemini_71

Gemini_72
(exonuclease)

Gemini_73

Gemini_74
(ERF family ssDNA binding protein)

Gemini_75

Gemini_76

Gemini_77

Gemini_78

Gemini_79

Gemini_80

Gemini_81
(DprA-like DNA processing chain A)

Gemini_82
(HNH endonuclease)

Gemini_83

Gemini_84

Gemini_85

Gemini_86
(DnaB-like dsDNA helicase)

Gemini_87

Gemini_88
(WhiB family transcription factor)

Gemini_89

Gemini_90

Gemini_91
(holliday junction resolvase)

Gemini_92
(membrane protein)

Gemini_93
(polynucleotide kinase)

Gemini_94

Gemini_95
(membrane protein)

Gemini_96
(RNA ligase)

Gemini_97
(helix-turn-helix DNA-binding domain protein)

Gemini_98
(WhiB family transcription factor)

Gemini_99

Gemini_100

Gemini_101

Gemini_102

Gemini_103

Gemini_104
(endonuclease VII)

Gemini_105
(ssDNA binding protein)

Gemini_106
(ClpP-like protease)

Gemini_107

Gemini_108

Gemini_109

Gemini_110
(helix-turn-helix DNA-binding domain protein)

Gemini_111
(HNH endonuclease)

Gemini_114

Gemini_115

Gemini_116

Gemini_117

Gemini_118
(DNA polymerase III subunit)

Gemini_119

Gemini_120
(RecA-like DNA recombinase)

Gemini_121

Gemini_122

Gemini_123

Gemini_124

Gemini_125

Gemini_126

Gemini_127

Gemini_128

Gemini_129

Gemini_130

Gemini_131

Gemini_132
(helix-turn-helix DNA-binding domain protein)

Gemini_133

Gemini_134

Gemini_135

Gemini_136

Gemini_137

Gemini_138

Gemini_139

Gemini_140

Gemini_141

Gemini_142

Gemini_143

Gemini_144

Gemini_145

Gemini_146

Gemini_147

Gemini_148

Gemini_149

Gemini_150

Gemini_151
(HNH endonuclease)

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMH779503
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Designator 2010WEKUgeminiEWW
SEA Lysate Titer 7.4e10
Date of SEA Lysate Titering Oct 5, 2010
Pitt Freezer Box# 20
Pitt Freezer Box Grid# 1
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload