Mycobacterium phage Kanye
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Detailed Information for Phage Kanye
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByAlexander Adia
Year Found2014
Location FoundProvidence, RI USA
Finding InstitutionBrown University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation TemperatureNot entered
GPS Coordinates41.824255 N, 71.403221 W Map
Discovery NotesThe soil sample was taken from a tree in front of Everett House on Keeney Quad. The soil was particularly moist because it had rained on Providence shortly before I collected my sample and because I collected my soil less than a foot from the base of the tree.
Naming NotesMy first thought when I saw my phage was that it had a big head. I also thought it would be a privilege to say that Kanye had, quite literally, gone viral.

While I was initially devastated that I couldn't come up with a pun involving phage due to the naming rules (Ellen Phage and Nacho Phage were casualties of such rules), Kanye was a very welcome consolation prize.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 28, 2015
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage1621
Genome length (bp)75453
Character of genome ends3' Sticky Overhang
Overhang Length9 bases
Overhang SequenceCGCTTGTCA
GC Content63.1%
Fasta file available?Yes: Download fasta file
Characterization
ClusterE
Subcluster--
Cluster Life CycleTemperate
Other Cluster Members
Annotating InstitutionPurdue University
Annotation StatusIn GenBank
Plaque NotesPlaques were always clear and and about 1-2 mm in size.
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List

Click to View

Kanye_1

Kanye_2

Kanye_3

Kanye_4

Kanye_5

Kanye_6
(terminase small subunit)

Kanye_7

Kanye_8

Kanye_9

Kanye_10
(terminase large subunit)

Kanye_11

Kanye_12
(portal protein)

Kanye_13
(capsid maturation protease)

Kanye_14
(major capsid protein)

Kanye_15

Kanye_16
(head-to-tail adaptor)

Kanye_17
(head-to-tail stopper)

Kanye_18

Kanye_19
(tail terminator)

Kanye_20
(major tail protein)

Kanye_21

Kanye_22
(tail assembly chaperone)

Kanye_23
(tail assembly chaperone)

Kanye_24
(tape measure protein)

Kanye_25
(minor tail protein)

Kanye_26
(minor tail protein)

Kanye_27
(minor tail protein)

Kanye_28
(minor tail protein)

Kanye_29
(minor tail protein)

Kanye_30
(minor tail protein)

Kanye_31

Kanye_32
(helix-turn-helix DNA binding domain protein)

Kanye_33

Kanye_34
(lysin A)

Kanye_35
(holin)

Kanye_36

Kanye_37
(lysin B)

Kanye_38

Kanye_39
(NrdH-like glutaredoxin)

Kanye_40
(Lsr2-like DNA bridging protein)

Kanye_41

Kanye_42

Kanye_43

Kanye_44

Kanye_45

Kanye_46

Kanye_47

Kanye_48
(tyrosine integrase)

Kanye_49

Kanye_50

Kanye_51
(immunity repressor)

Kanye_52

Kanye_53

Kanye_54
(pentapeptide repeat protein)

Kanye_55

Kanye_56

Kanye_57

Kanye_58

Kanye_59

Kanye_60

Kanye_61

Kanye_62

Kanye_63

Kanye_64

Kanye_65

Kanye_66
(ERF family ssDNA binding protein)

Kanye_67

Kanye_68

Kanye_69

Kanye_70

Kanye_71

Kanye_72

Kanye_73
(DprA-like DNA processing chain A)

Kanye_74
(HNH endonuclease)

Kanye_75

Kanye_76

Kanye_77

Kanye_78
(DnaB-like dsDNA helicase)

Kanye_79

Kanye_80
(WhiB family transcription factor)

Kanye_81

Kanye_82

Kanye_83
(holliday junction resolvase)

Kanye_84

Kanye_85
(polynucleotide kinase)

Kanye_86

Kanye_87

Kanye_88
(RNA ligase)

Kanye_89
(helix-turn-helix DNA binding domain protein)

Kanye_90
(WhiB family transcription factor)

Kanye_91

Kanye_92

Kanye_93

Kanye_94

Kanye_95

Kanye_96
(DNA methylase)

Kanye_97
(ssDNA binding protein)

Kanye_98
(ClpP-like protease)

Kanye_99

Kanye_100

Kanye_101

Kanye_102
(helix-turn-helix DNA binding domain protein)

Kanye_103
(HNH endonuclease)

Kanye_106

Kanye_107

Kanye_108
(hydrolase)

Kanye_109

Kanye_110
(DNA polymerase III subunit)

Kanye_111

Kanye_112
(RecA-like DNA recombinase)

Kanye_113

Kanye_114

Kanye_115

Kanye_116

Kanye_117

Kanye_118

Kanye_119

Kanye_120

Kanye_121

Kanye_122

Kanye_123

Kanye_124
(helix-turn-helix DNA binding domain protein)

Kanye_125

Kanye_126

Kanye_127

Kanye_128

Kanye_129

Kanye_130

Kanye_131

Kanye_132

Kanye_133

Kanye_134

Kanye_135

Kanye_136

Kanye_137

Kanye_138

Kanye_139

Kanye_140

Kanye_141

Kanye_142
(HNH endonuclease)

Submitted Annotation in DNA Master FormatDownload
Submitted Minimal DNA Master FileDownload
Author ListDownload
Annotation Cover SheetDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMN428059
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Lysate Titer 2.6x10^10 pfus/mL
Date of SEA Lysate Titering Dec 3, 2014
Pitt Freezer Box# 11
Pitt Freezer Box Grid# F5
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload