Mycobacterium phage Kanye
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Detailed Information for Phage Kanye
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByAlexander Adia
Year Found2014
Location FoundProvidence, RI United States
Finding InstitutionBrown University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation TemperatureNot entered
GPS Coordinates41.824255 N, 71.403221 W Map
Discovery NotesThe soil sample was taken from a tree in front of Everett House on Keeney Quad. The soil was particularly moist because it had rained on Providence shortly before I collected my sample and because I collected my soil less than a foot from the base of the tree.
Naming NotesMy first thought when I saw my phage was that it had a big head. I also thought it would be a privilege to say that Kanye had, quite literally, gone viral.

While I was initially devastated that I couldn't come up with a pun involving phage due to the naming rules (Ellen Phage and Nacho Phage were casualties of such rules), Kanye was a very welcome consolation prize.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 28, 2015
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage1621
Genome length (bp)75453
Character of genome ends3' Sticky Overhang
Overhang Length9 bases
Overhang SequenceCGCTTGTCA
GC Content63.1%
Fasta file available?Yes: Download fasta file
Cluster Life CycleTemperate
Other Cluster Members
Annotation StatusPlanless
Plaque NotesPlaques were always clear and and about 1-2 mm in size.
Has been Phamerated?Yes
Gene List
Publication Info
Uploaded to GenBank?No
GenBank AccessionNone yet
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Lysate Titer 2.6x10^10 pfus/mL
Date of SEA Lysate Titering Dec 3, 2014
Pitt Freezer Box# 11
Pitt Freezer Box Grid# F5
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload