| Detailed Information for Phage Sassay |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Team M.C. Smegger |
| Year Found | 2014 |
| Location Found | Los Angeles, CA USA |
| Finding Institution | University of California, Los Angeles |
| Program | Unavailable |
| From enriched soil sample? | Yes |
| Isolation Temperature | 35°C |
| GPS Coordinates | 34.07 N, 118.452 W Map |
| Discovery Notes | Phage isolation performed in Spring 2014 and genome annotation performed in Fall 2014 by Team M.C. Smegger: Meghana Attaluri, Mark LaVigne, Saul Lincoln, Cydney Nichols. Annotation was quality checked by Edric Yoon and Lei Yeh. TA: Emma Goodwin. DNA preparation performed by Dr. Krisanavane Reddi (UCLA). Assembly performed by Dr. Jordan Moberg Parker (UCLA).
Sample description: Sample collected from moist soil from a flower bed by Cydney Nichols on April 4, 2014.
3 cm deep
Temperature: 15.56oC
Urban environment close to foot/vehicle traffic |
| Naming Notes | Phage Sassay (combination of “sassy” and the commonly used lab term “assay”) |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 23, 2015 |
| Sequencing Facility | The UCLA Genotyping and Sequencing Core |
| Shotgun Sequencing Method | 454 Pyrosequencing |
| Approximate Shotgun Coverage | 126 |
| Genome length (bp) | 73495 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 9 bases |
| Overhang Sequence | CGCTTGTCA |
| GC Content | 63.0% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | E |
| Subcluster | -- |
| Cluster Life Cycle | Temperate |
| Lysogeny Notes | Plaque morphology turbid with halos, genome contains integrase. |
| Other Cluster Members |
|
| Annotating Institution | Unknown or unassigned |
| Annotation Status | In GenBank |
| Plaque Notes | Turbid, with halos occasionally, 2mm in radius |
| Morphotype | Siphoviridae |
| Number of Genes | 139 |
| Number of tRNAs | 2 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MF919529 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
23 |
| Pitt Freezer Box Grid# |
D12 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
