Detailed Information for Phage Ukulele |
Discovery Information |
Isolation Host | Mycobacterium smegmatis mc²155 |
Found By | Maryanne LaFollette and Matthew Sheltra |
Year Found | 2011 |
Location Found | Old Orchard Beach, ME USA |
Finding Institution | University of Maine, Honors College |
Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
From enriched soil sample? | Yes |
Isolation Temperature | Not entered |
GPS Coordinates | 43.517800 N, 70.377300 W Map |
Discovery Notes | Soil sample was found in a flower garden ~2 cm deep. Temperature was approximately 69?, and soil was slightly damp. |
Sequencing Information |
Sequencing Complete? | Yes |
Date Sequencing Completed | Jan 31, 2012 |
Sequencing Facility | Pittsburgh Bacteriophage Institute |
Shotgun Sequencing Method | Ion Torrent |
Approximate Shotgun Coverage | 133 |
Genome length (bp) | 75114 |
Character of genome ends | 3' Sticky Overhang |
Overhang Length | 9 bases |
Overhang Sequence | CGCTTGTCA |
GC Content | 63.0% |
Fasta file available? | Yes: Download fasta file |
Characterization |
Cluster | E |
Subcluster | -- |
Cluster Life Cycle | Temperate |
Other Cluster Members |
|
Annotating Institution | Unknown or unassigned |
Annotation Status | In GenBank |
Plaque Notes | Plaque morphology ranges from ~0.5 mm to ~2.0 mm, and is slightly turbid. |
Morphotype | Siphoviridae |
Number of Genes | 141 |
Number of tRNAs | 2 |
Number of tmRNAs | 0 |
Has been Phamerated? | Yes |
Gene List |
|
Submitted Annotation in DNA Master Format | Download |
Author List | Download |
Annotation Cover Sheet | Download |
Publication Info |
Uploaded to GenBank? | Yes |
GenBank Accession | KT373978 |
Refseq Number | None yet |
Published in Paper | Pope, Bowman, Russell, et al 2015, eLIFE: Whole genome comparison of a large collection of mycobacteriophages reveals a continuum of phage genetic diversity |
Archiving Info |
Archiving status |
Archived |
Pitt Freezer Box# |
25 |
Pitt Freezer Box Grid# |
13 |
Available Files |
Plaque Picture | Download |
Restriction Digest Picture | Download |
EM Picture | Download |
Final DNAMaster File | Download |