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Mycobacterium phage Thresher
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Detailed Information for Phage Thresher
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByCydne Kitchens
Year Found2016
Location FoundBowling Green, KY USA
Finding InstitutionWestern Kentucky University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature30°C
GPS Coordinates37.008335 N, 86.376597 W Map
Discovery NotesThe soil sample was taken from the side of the road next to a branch of Fruit of the Loom.
Naming NotesIt was named after my favorite species of shark. This species of shark is known for having an exceptionally long caudal fin, similar to the long tail of a siphoviridae.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedFeb 17, 2021
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage185
Genome length (bp)76192
Character of genome ends3' Sticky Overhang
Overhang Length9 bases
Overhang SequenceCGCTTGTCA
GC Content63.0%
Fasta file available?Yes: Download fasta file
Characterization
ClusterE
Subcluster--
Cluster Life CycleTemperate
Other Cluster Members
Annotating InstitutionWestern Kentucky University
Annotation StatusIn GenBank
Plaque NotesThis phage produced clear plaques. This made it easier to see, work with, and count.
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List

Click to View

Thresher_1

Thresher_2

Thresher_3

Thresher_4

Thresher_5

Thresher_6
(terminase small subunit)

Thresher_7

Thresher_8

Thresher_9
(terminase large subunit)

Thresher_10

Thresher_11
(portal protein)

Thresher_12
(capsid maturation protease)

Thresher_13
(major capsid protein)

Thresher_14

Thresher_15
(head-to-tail adaptor)

Thresher_16
(head-to-tail stopper)

Thresher_17

Thresher_18
(tail terminator)

Thresher_19
(major tail protein)

Thresher_20
(head fiber protein)

Thresher_21
(tail assembly chaperone)

Thresher_22
(tail assembly chaperone)

Thresher_23
(tape measure protein)

Thresher_24
(minor tail protein)

Thresher_25
(minor tail protein)

Thresher_26
(minor tail protein)

Thresher_27
(minor tail protein)

Thresher_28
(minor tail protein)

Thresher_29

Thresher_30

Thresher_31
(helix-turn-helix DNA binding domain protein)

Thresher_32

Thresher_33
(lysin A)

Thresher_34
(holin)

Thresher_35

Thresher_36
(lysin B)

Thresher_37

Thresher_38
(glutaredoxin)

Thresher_39

Thresher_40
(Lsr2-like DNA bridging protein)

Thresher_41

Thresher_42

Thresher_43

Thresher_44

Thresher_45

Thresher_46

Thresher_47

Thresher_48

Thresher_49

Thresher_50

Thresher_51
(methyltransferase)

Thresher_52

Thresher_53

Thresher_54

Thresher_55
(tyrosine integrase)

Thresher_56

Thresher_57

Thresher_58
(immunity repressor)

Thresher_59

Thresher_60

Thresher_61
(pentapeptide repeat protein)

Thresher_62

Thresher_63

Thresher_64

Thresher_65

Thresher_66

Thresher_67

Thresher_68

Thresher_69
(exonuclease)

Thresher_70

Thresher_71
(ERF family ssDNA binding protein)

Thresher_72

Thresher_73

Thresher_74

Thresher_75

Thresher_76

Thresher_77

Thresher_78
(DprA-like DNA processing chain A)

Thresher_79
(HNH endonuclease)

Thresher_80

Thresher_81

Thresher_82

Thresher_83
(DnaB-like dsDNA helicase)

Thresher_84

Thresher_85
(WhiB family transcription factor)

Thresher_86

Thresher_87

Thresher_88
(holliday junction resolvase)

Thresher_89

Thresher_90
(polynucleotide kinase)

Thresher_91

Thresher_92

Thresher_93

Thresher_94
(RNA ligase)

Thresher_95
(helix-turn-helix DNA binding domain protein)

Thresher_96
(WhiB family transcription factor)

Thresher_97

Thresher_98

Thresher_99

Thresher_100

Thresher_101
(DNA methylase)

Thresher_102
(ssDNA binding protein)

Thresher_103
(ClpP-like protease)

Thresher_104

Thresher_105

Thresher_106
(helix-turn-helix DNA binding domain protein)

Thresher_107
(HNH endonuclease)

Thresher_110

Thresher_111

Thresher_112

Thresher_113

Thresher_114
(DnaQ-like DNA polymerase III subunit)

Thresher_115

Thresher_116
(RecA-like DNA recombinase)

Thresher_117

Thresher_118

Thresher_119

Thresher_120

Thresher_121

Thresher_122

Thresher_123

Thresher_124

Thresher_125

Thresher_126

Thresher_127

Thresher_128
(helix-turn-helix DNA binding domain protein)

Thresher_129

Thresher_130

Thresher_131

Thresher_132

Thresher_133

Thresher_134

Thresher_135

Thresher_136

Thresher_137

Thresher_138

Thresher_139

Thresher_140

Thresher_141

Thresher_142

Thresher_143

Thresher_144

Thresher_145

Thresher_146

Thresher_147

Thresher_148
(HNH endonuclease)

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMZ622158
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Designator 2016WEKUtresherCK
SEA Lysate Titer 1.3 x 10^10 pfu/mL
Pitt Freezer Box# 37
Pitt Freezer Box Grid# D9
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload