Mycobacterium phage Thresher
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Detailed Information for Phage Thresher
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByCydne Kitchens
Year Found2016
Location FoundBowling Green, KY USA
Finding InstitutionWestern Kentucky University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature30°C
GPS Coordinates37.008335 N, 86.376597 W Map
Discovery NotesThe soil sample was taken from the side of the road next to a branch of Fruit of the Loom.
Naming NotesIt was named after my favorite species of shark. This species of shark is known for having an exceptionally long caudal fin, similar to the long tail of a siphoviridae.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedFeb 17, 2021
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage185
Genome length (bp)76192
Character of genome ends3' Sticky Overhang
Overhang Length9 bases
Overhang SequenceCGCTTGTCA
GC Content63.0%
Fasta file available?Yes: Download fasta file
Characterization
ClusterE
Subcluster--
Cluster Life CycleTemperate
Other Cluster Members
Annotating InstitutionWestern Kentucky University
Annotation StatusBeing Annotated (Expected completion by 5/1/2021)
Plaque NotesThis phage produced clear plaques. This made it easier to see, work with, and count.
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List

Click to View

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Publication Info
Uploaded to GenBank?No
GenBank AccessionNone yet
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Designator 2016WEKUtresherCK
SEA Lysate Titer 1.3 x 10^10 pfu/mL
Pitt Freezer Box# 37
Pitt Freezer Box Grid# D9
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload