| Detailed Information for Phage MISSy |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Team Gone Viral |
| Year Found | 2014 |
| Location Found | Los Angeles, CA USA |
| Finding Institution | University of California, Los Angeles |
| Program | Unavailable |
| From enriched soil sample? | Yes |
| Isolation Temperature | 35°C |
| GPS Coordinates | 34.055939 N, 118.4424 W Map |
| Discovery Notes | Phage isolation performed in Spring 2014 and genome annotation performed in Fall 2014 by Team Gone Viral: Michelle Liao, Iger Ostreni, Sara Komenan, Shayli Swaim. Annotation quality checked by Edric Yoon, Lei Yeh, Iger Ostreni and Emily Xu. DNA preparation performed by Dr. Krisanavane Reddi (UCLA). Assembly performed by Dr. Jordan Moberg Parker (UCLA).
Sample description: Soil from planter in an urban area with low foot traffic outside of Roberts Luxury Apartments on April 2, 2014.
Depth from which sample was taken (in inches or cm): 0-3cm
Air temperature if sample collected outdoors (in degrees Celsius): 14oC
Additional information of interest or note: There was 0.25 inches of precipitation the night before. |
| Naming Notes | This name was picked from the first letters of the names of each person on the team: Michelle, Iger, Sara, and Shayli. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 23, 2015 |
| Sequencing Facility | The UCLA Genotyping and Sequencing Core |
| Shotgun Sequencing Method | 454 Pyrosequencing |
| Approximate Shotgun Coverage | 63 |
| Genome length (bp) | 75808 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 9 bases |
| Overhang Sequence | CGCTTGTCA |
| GC Content | 63.1% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | E |
| Subcluster | -- |
| Cluster Life Cycle | Temperate |
| Lysogeny Notes | Evidence for lysogeny: bulls-eye plaques and genome contains integrase |
| Other Cluster Members |
|
| Annotating Institution | Unknown or unassigned |
| Annotation Status | In GenBank |
| Plaque Notes | Bulls-eye morphology with clear inner circle, turbid middle ring, clear outer ring. Average diameter of ~4.5 ± 0.58 mm. Size range of 3.5-6.0mm. |
| Morphotype | Siphoviridae |
| Number of Genes | 127 |
| Number of tRNAs | 2 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MF919524 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
23 |
| Pitt Freezer Box Grid# |
D8 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
