The Actinobacteriophage Database

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Detailed Information for Phage Murphy
Discovery Information
Isolation Host	Mycobacterium smegmatis mc²155
Found By	Michael Anderson
Year Found	2011
Location Found	Columbus, OH USA
Finding Institution	The Ohio State University
Program	Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?	Yes
Isolation Temperature	Not entered
GPS Coordinates	40.004213 N, 83.021904 W Map
Discovery Notes	Very wet and muddy. On the bank of the Olentangy River, underneath Woody Hayes Bridge in Columbus Ohio. Taken from 1 inch under the surface. Shaded all day.
Sequencing Information
Sequencing Complete?	Yes
Date Sequencing Completed	Jan 24, 2012
Sequencing Facility	Virginia Commonwealth University Nucleic Acids Research Facilities
Shotgun Sequencing Method	454 Pyrosequencing
Approximate Shotgun Coverage	170
Genome length (bp)	76179
Character of genome ends	3' Sticky Overhang
Overhang Length	9 bases
Overhang Sequence	CGCTTGTCA
GC Content	62.9%
Fasta file available?	Yes: Download fasta file
Characterization
Cluster	E
Subcluster	--
Cluster Life Cycle	Temperate
Other Cluster Members	Click to View 244 ABCat Adnama Amao AmericanBeauty Argent26 Asriel BaboJay BadStone Balomoji Barbarian Bask21 Bench BigBubba BilboSwaggins Bruin Buck Cactus ChosenOne ChotaBhai Cjw1 Command613 Contagion Cookies CrystalP Czyszczon1 Daikon DoctorDiddles DrDrey Dumbo Dusk Easy2Say Elite2014 Elph10 Emmina Eureka Filch FireRed Flypotenuse Gage Gator Gemini Glexan Goku GoldenSpark Goldilocks GooberAzure HanKaySha Harella Henry Highbury Holt Hoonter Hopey HufflyPuff Icee IHOP Inca JeTaime Kanye Kimchi Kostya Lilac Lilizi Lilpickle MadamMonkfish Manda Marshmallow Maxxinista Mindy Miniwave Misfit MISSy Moldemort Mosby MPhalcon Murica Murphy Myrale Nala NelitzaMV Nimrod NoSleep OrionPax Paperbeatsrock Pat3 Petra64142 Phaja Pharsalus PhatBacter Phaux Phrux Porky Pumpkin Quallification Rakim Rimmer RiverMonster Saints25 Sassay ShamWow ShereKhan Simpliphy SirDuracell SophKB Sotrice96 Stank Stark StellaBean StolenFromERC Tarkin TBrady12 TeardropMSU Teaspoon Terminus Thresher Tomaszewski Toto Traaww1 Tuco Ukulele Wiggin Willez Xandras xkcd YassJohnny Youngblood
Annotating Institution	Unknown or unassigned
Annotation Status	In GenBank
Plaque Notes	2-3mm in diameter. Whole in the center that fades out towards the edge.
Morphotype	Siphoviridae
Has been Phamerated?	Yes
Gene List	Click to View Murphy_1 Murphy_2 Murphy_3 Murphy_4 Murphy_5 (terminase small subunit) Murphy_6 Murphy_7 Murphy_8 (terminase large subunit) Murphy_9 Murphy_10 (portal protein) Murphy_11 (capsid maturation protease) Murphy_12 (major capsid protein) Murphy_13 Murphy_14 (head-to-tail adaptor) Murphy_15 (head-to-tail stopper) Murphy_16 Murphy_17 (tail terminator) Murphy_18 (major tail subunit) Murphy_19 Murphy_20 (tail assembly chaperone) Murphy_21 (tail assembly chaperone) Murphy_22 (HNH endonuclease) Murphy_23 (tape measure protein) Murphy_24 (minor tail protein) Murphy_25 (minor tail protein) Murphy_26 (minor tail protein) Murphy_27 (minor tail protein) Murphy_28 (D-Ala-D-Ala carboxypeptidase) Murphy_29 Murphy_30 Murphy_31 Murphy_32 Murphy_33 (lysin A) Murphy_34 (holin) Murphy_35 Murphy_36 (lysin B) Murphy_37 Murphy_38 (NrdH-like protein) Murphy_39 (Lsr2) Murphy_40 Murphy_41 Murphy_42 Murphy_43 Murphy_44 Murphy_45 Murphy_46 Murphy_47 Murphy_48 Murphy_49 Murphy_50 Murphy_51 Murphy_52 Murphy_53 Murphy_54 (tyrosine integrase) Murphy_55 Murphy_56 Murphy_57 (immunity repressor) Murphy_58 Murphy_59 Murphy_60 (pentapeptide repeat domain protein) Murphy_61 Murphy_62 Murphy_63 Murphy_64 Murphy_65 Murphy_66 Murphy_67 Murphy_68 Murphy_69 (ssDNA binding protein, ERF family) Murphy_70 Murphy_71 Murphy_72 Murphy_73 Murphy_74 Murphy_75 Murphy_76 Murphy_77 (HNH endonuclease domain protein) Murphy_78 Murphy_79 Murphy_80 Murphy_81 (DnaB-like helicase) Murphy_82 Murphy_83 (WhiB family transcription factor) Murphy_84 Murphy_85 Murphy_86 Murphy_87 Murphy_88 (polynucleotide kinase) Murphy_89 Murphy_90 Murphy_91 (RNA ligase) Murphy_92 Murphy_93 (WhiB) Murphy_94 Murphy_95 Murphy_96 Murphy_97 Murphy_98 Murphy_99 (DNA methylase) Murphy_100 (ssDNA binding protein) Murphy_101 (ClpP-like protease) Murphy_102 Murphy_103 Murphy_104 Murphy_105 (HNH homing endonuclease domain protein) Murphy_108 Murphy_109 Murphy_110 Murphy_111 Murphy_112 (DnaQ-like DNA polymerase III subunit) Murphy_113 Murphy_114 (RecA-like DNA recombinase) Murphy_115 Murphy_116 Murphy_117 Murphy_118 Murphy_119 Murphy_120 Murphy_121 Murphy_122 Murphy_123 Murphy_124 Murphy_125 Murphy_126 Murphy_127 Murphy_128 Murphy_129 Murphy_130 Murphy_131 Murphy_132 Murphy_133 Murphy_134 Murphy_135 Murphy_136 Murphy_137 Murphy_138 Murphy_139 Murphy_140 Murphy_141 Murphy_142 Murphy_143 Murphy_144 Murphy_145 Murphy_146 Murphy_147 Murphy_148 (HNH endonuclease domain protein)
Publication Info
Uploaded to GenBank?	Yes
GenBank Accession	KC748971
Refseq Number	NC_021305
Published in Paper	Pope, Bowman, Russell, et al 2015, eLIFE: Whole genome comparison of a large collection of mycobacteriophages reveals a continuum of phage genetic diversity
Archiving Info
Archiving status	Archived
SEA Designator	OHSU
Pitt Freezer Box#	26
Pitt Freezer Box Grid#	71
Available Files
Plaque Picture	Download

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