The Actinobacteriophage Database

Mycobacterium phage Elph10

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Detailed Information for Phage Elph10
Discovery Information
Isolation Host	Mycobacterium smegmatis mc²155
Former names	Elphonso10
Found By	Adam Groth
Year Found	2010
Location Found	River Falls, WI USA
Finding Institution	University of Wisconsin-River Falls
Program	Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?	Yes
Isolation Temperature	Not entered
GPS Coordinates	44.851678 N, 92.623619 W Map
Discovery Notes	Depth: 4cm Air temp: 60°F Soil Type: slightly sandy
Sequencing Information
Sequencing Complete?	Yes
Date Sequencing Completed	Jan 28, 2011
Genome length (bp)	74675
Character of genome ends	3' Sticky Overhang
Overhang Length	9 bases
Overhang Sequence	CGCTTGTCA
GC Content	63.0%
Fasta file available?	Yes: Download fasta file
Characterization
Cluster	E
Subcluster	--
Cluster Life Cycle	Temperate
Other Cluster Members	Click to View 244 ABCat Adnama Amao AmericanBeauty Argent26 Asriel BaboJay BadStone Barbarian Bask21 Bench BilboSwaggins Bruin Buck Cactus ChosenOne ChotaBhai Cjw1 Command613 Contagion Cookies CrystalP Czyszczon1 DoctorDiddles DrDrey Dumbo Dusk Easy2Say Elite2014 Elph10 Emmina Eureka Filch FireRed Flypotenuse Gage Gator Gemini Glexan Goku GoldenSpark Goldilocks GooberAzure HanKaySha Harella Henry Highbury Holt Hoonter Hopey HufflyPuff Icee IHOP Inca JeTaime Kanye Kimchi Kostya Lilac Lilizi Lilpickle MadamMonkfish Manda Marshmallow Maxxinista Mindy Miniwave Misfit MISSy Moldemort Mosby MPhalcon Murica Murphy Myrale Nala NelitzaMV Nimrod NoSleep OrionPax Paperbeatsrock Pat3 Petra64142 Phaja Pharsalus PhatBacter Phaux Phrux Porky Pumpkin Quallification Rakim Rimmer RiverMonster Saints25 Sassay ShamWow ShereKhan Simpliphy SirDuracell SophKB Sotrice96 Stark StolenFromERC Tarkin TBrady12 TeardropMSU Terminus Thresher Tomaszewski Toto Traaww1 Tuco Ukulele Wiggin Willez xkcd YassJohnny Youngblood
Annotating Institution	Unknown or unassigned
Annotation Status	In GenBank
Plaque Notes	Size: 1mm in diameter Clear Halos formed after 48 hours of incubation
Morphotype	Siphoviridae
Number of Genes	143
Number of tRNAs	2
Has been Phamerated?	Yes
Gene List	Click to View Elph10_1 Elph10_2 Elph10_3 Elph10_4 Elph10_5 Elph10_6 (terminase small subunit) Elph10_7 Elph10_8 Elph10_9 (terminase large subunit) Elph10_10 Elph10_11 (portal protein) Elph10_12 (capsid maturation protease) Elph10_13 (major capsid protein) Elph10_14 Elph10_15 (head-to-tail adaptor) Elph10_16 (head-to-tail stopper) Elph10_17 Elph10_18 (tail terminator) Elph10_19 (major tail subunit) Elph10_20 Elph10_21 (tail assembly chaperone) Elph10_22 (tail assembly chaperone) Elph10_23 (tape measure protein) Elph10_24 (minor tail subunit) Elph10_25 (minor tail subunit) Elph10_26 (minor tail subunit) Elph10_27 (minor tail subunit) Elph10_28 (D-Ala-D-Ala carboxypeptidase) Elph10_29 Elph10_30 Elph10_31 Elph10_32 Elph10_33 (lysin A) Elph10_34 (holin) Elph10_35 Elph10_36 (lysin B) Elph10_37 Elph10_38 (NrdH) Elph10_39 (Lsr2) Elph10_40 Elph10_41 Elph10_42 Elph10_43 Elph10_44 Elph10_45 Elph10_46 Elph10_47 Elph10_48 Elph10_49 Elph10_50 Elph10_51 Elph10_52 (tyrosine integrase) Elph10_53 Elph10_54 Elph10_55 Elph10_56 Elph10_57 Elph10_58 (pentapeptide repeat family protein) Elph10_59 Elph10_60 Elph10_61 Elph10_62 Elph10_63 Elph10_64 Elph10_65 Elph10_66 Elph10_67 Elph10_68 Elph10_69 (ssDNA binding protein, ERF family) Elph10_70 Elph10_71 Elph10_72 Elph10_73 Elph10_74 Elph10_75 Elph10_76 Elph10_77 (HNH endonuclease domain protein) Elph10_78 Elph10_79 Elph10_80 Elph10_81 (DnaB-like helicase) Elph10_82 Elph10_83 Elph10_84 Elph10_85 Elph10_86 Elph10_87 Elph10_88 (Pnk) Elph10_89 Elph10_90 Elph10_91 Elph10_92 (RNA ligase) Elph10_93 Elph10_94 (WhiB) Elph10_95 Elph10_96 Elph10_97 Elph10_98 Elph10_99 (DNA methyltransferase) Elph10_100 (ssDNA binding protein) Elph10_101 (Clp protease) Elph10_102 Elph10_103 Elph10_104 Elph10_105 Elph10_108 Elph10_109 Elph10_110 Elph10_111 Elph10_112 (DnaQ-like exonuclease) Elph10_113 Elph10_114 (RecA) Elph10_115 Elph10_116 Elph10_117 Elph10_118 Elph10_119 Elph10_120 Elph10_121 Elph10_122 Elph10_123 Elph10_124 Elph10_125 Elph10_126 (ParBc domain protein) Elph10_127 Elph10_128 Elph10_129 Elph10_130 Elph10_131 Elph10_132 Elph10_133 Elph10_134 Elph10_135 Elph10_136 Elph10_137 Elph10_138 Elph10_139 Elph10_140 Elph10_141 Elph10_142 Elph10_143 Elph10_144 Elph10_145
Publication Info
Uploaded to GenBank?	Yes
GenBank Accession	JN391441
Refseq Number	None yet
Published in Paper	Pope, Bowman, Russell, et al 2015, eLIFE: Whole genome comparison of a large collection of mycobacteriophages reveals a continuum of phage genetic diversity
Archiving Info
Archiving status	Archived
SEA Designator	2010UWRFElph10AMG
SEA Lysate Titer	4.2e10
Date of SEA Lysate Titering	Oct 20, 2010
Pitt Freezer Box#	1
Pitt Freezer Box Grid#	G3
Available Files
Plaque Picture	Download
Restriction Digest Picture	Download
EM Picture	Download
Final DNAMaster File	Download

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