Mycobacterium phage Flypotenuse
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Detailed Information for Phage Flypotenuse
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByAlexis Jackson
Year Found2018
Location FoundBlacksburg, VA USA
Finding InstitutionVirginia Tech
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature37°C
GPS Coordinates37.2284 N, 80.4248 W Map
Discovery Notesfound on a dirt path which has heavy traffic between two academic buildings at Virginia Tech ( Robeson and Pamplin)
Naming NotesThe name came from the word Hypotenuse and Fly. Hypotenuse came from the fact it was a long tail and fly because I believed it was a cool phage.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedNov 30, 2018
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage2017
Genome length (bp)75248
Character of genome ends3' Sticky Overhang
Overhang Length9 bases
Overhang SequenceCGCTTGTCA
GC Content67.3%
Fasta file available?Yes: Download fasta file
Characterization
ClusterE
Subcluster--
Cluster Life CycleTemperate
Other Cluster Members
Annotating InstitutionVirginia Tech
Annotation StatusIn GenBank
Plaque NotesPlaques were clear with a barely noticeable halo around it.
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List

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Flypotenuse_1

Flypotenuse_2

Flypotenuse_3

Flypotenuse_4

Flypotenuse_5
(terminase small subunit)

Flypotenuse_6

Flypotenuse_7
(terminase large subunit)

Flypotenuse_8

Flypotenuse_9
(portal protein)

Flypotenuse_10
(capsid maturation protease)

Flypotenuse_11
(major capsid protein)

Flypotenuse_12

Flypotenuse_13
(head-to-tail adaptor)

Flypotenuse_14
(head-to-tail stopper)

Flypotenuse_15

Flypotenuse_16
(tail terminator)

Flypotenuse_17
(major tail protein)

Flypotenuse_18
(tail assembly chaperone)

Flypotenuse_19
(tail assembly chaperone)

Flypotenuse_20
(tape measure protein)

Flypotenuse_21
(minor tail protein)

Flypotenuse_22
(minor tail protein)

Flypotenuse_23
(minor tail protein)

Flypotenuse_24
(minor tail protein)

Flypotenuse_25
(minor tail protein)

Flypotenuse_26
(minor tail protein)

Flypotenuse_27

Flypotenuse_28
(helix-turn-helix DNA binding domain protein)

Flypotenuse_29

Flypotenuse_30
(lysin A)

Flypotenuse_31
(holin)

Flypotenuse_32

Flypotenuse_33
(lysin B)

Flypotenuse_34

Flypotenuse_35
(NrdH-like glutaredoxin)

Flypotenuse_36
(Lsr2-like DNA bridging protein)

Flypotenuse_37

Flypotenuse_38

Flypotenuse_39

Flypotenuse_40

Flypotenuse_41

Flypotenuse_42

Flypotenuse_43

Flypotenuse_44

Flypotenuse_45

Flypotenuse_46

Flypotenuse_47
(tyrosine integrase)

Flypotenuse_48

Flypotenuse_49
(membrane protein)

Flypotenuse_50
(immunity repressor)

Flypotenuse_51

Flypotenuse_52

Flypotenuse_53
(pentapeptide repeat protein)

Flypotenuse_54

Flypotenuse_55

Flypotenuse_56

Flypotenuse_57

Flypotenuse_58

Flypotenuse_59

Flypotenuse_60

Flypotenuse_61

Flypotenuse_62

Flypotenuse_63

Flypotenuse_64

Flypotenuse_65
(ERF family ssDNA binding protein)

Flypotenuse_66

Flypotenuse_67

Flypotenuse_68

Flypotenuse_69

Flypotenuse_70

Flypotenuse_71

Flypotenuse_72

Flypotenuse_73
(HNH endonuclease)

Flypotenuse_74

Flypotenuse_75

Flypotenuse_76

Flypotenuse_77
(DnaB-like dsDNA helicase)

Flypotenuse_78

Flypotenuse_79
(WhiB family transcription factor)

Flypotenuse_80

Flypotenuse_81

Flypotenuse_82

Flypotenuse_83

Flypotenuse_84
(polynucleotide kinase)

Flypotenuse_85

Flypotenuse_86

Flypotenuse_87
(RNA ligase)

Flypotenuse_88
(helix-turn-helix DNA binding domain protein)

Flypotenuse_89
(WhiB family transcription factor)

Flypotenuse_90

Flypotenuse_91

Flypotenuse_92

Flypotenuse_93

Flypotenuse_94

Flypotenuse_95
(endonuclease VII)

Flypotenuse_96
(ssDNA binding protein)

Flypotenuse_97
(ClpP-like protease)

Flypotenuse_98

Flypotenuse_99

Flypotenuse_100

Flypotenuse_101

Flypotenuse_102
(HNH endonuclease)

Flypotenuse_105

Flypotenuse_106

Flypotenuse_107

Flypotenuse_108

Flypotenuse_109

Flypotenuse_110
(DNA polymerase III subunit)

Flypotenuse_111

Flypotenuse_112
(RecA-like DNA recombinase)

Flypotenuse_113

Flypotenuse_114

Flypotenuse_115

Flypotenuse_116

Flypotenuse_117

Flypotenuse_118

Flypotenuse_119

Flypotenuse_120

Flypotenuse_121

Flypotenuse_122

Flypotenuse_123
(helix-turn-helix DNA binding domain protein)

Flypotenuse_124

Flypotenuse_125

Flypotenuse_126

Flypotenuse_127

Flypotenuse_128

Flypotenuse_129

Flypotenuse_130

Flypotenuse_131

Flypotenuse_132

Flypotenuse_133

Flypotenuse_134

Flypotenuse_135

Flypotenuse_136

Flypotenuse_137

Flypotenuse_138

Flypotenuse_139

Flypotenuse_140

Flypotenuse_141

Flypotenuse_142
(HNH endonuclease)

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMK937593
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 80
Pitt Freezer Box Grid# B7
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload