Mycobacterium phage Filch
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Detailed Information for Phage Filch
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByPhilip Versluis
Year Found2014
Location FoundIL
Finding InstitutionHope College
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?No
Isolation TemperatureNot entered
GPS Coordinates42.266319 N, 88.024447 W Map
Naming NotesThe name Filch was given because none of the environmental samples that had been personally collected produced an isolated phage. An extra plate from a colleague that yielded plaques was "filched" with permission and purification proceeded.
Filch is also the nickname given to a past dog.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedAug 4, 2020
Sequencing FacilityHope College
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage4944
Genome length (bp)75692
Character of genome ends3' Sticky Overhang
Overhang Length9 bases
Overhang SequenceCGCTTGTCA
GC Content62.9%
Fasta file available?Yes: Download fasta file
Characterization
ClusterE
Subcluster--
Cluster Life CycleTemperate
Lysogeny NotesFilch is a temperate phage, although the plaque morphology produced is mostly clear; Filch is capable of producing a stable lysogen.
Other Cluster Members
Annotating InstitutionUniversity of the Sciences in Philadelphia
Annotation StatusIn GenBank
Plaque NotesFilch was isolated, and purified at 32 degrees Celsius. After 24 hours Filch exhibited clear plaques with diameter just below one millimeter. Filch continued to grow until 72 hours when it attained a plaque 3-4 millimeters in diameter.
After 48 hours of incubation, a turbid half ring appeared around the clear plaque.

Filch was incubated at 42 and 32 degrees Celsius. Plaques grew at 32 as well as 42, though Filch may be sensitive to the higher temperature.
Has been Phamerated?Yes
Gene List

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Filch_1

Filch_2

Filch_3

Filch_4

Filch_5

Filch_6
(terminase, small subunit)

Filch_7

Filch_8

Filch_9
(terminase, large subunit)

Filch_10

Filch_11
(portal protein)

Filch_12
(capsid maturation protease)

Filch_13
(major capsid protein)

Filch_14

Filch_15
(head-to-tail adaptor)

Filch_16
(head-to-tail stopper)

Filch_17

Filch_18
(tail terminator)

Filch_19
(major tail protein)

Filch_20

Filch_21
(tail assembly chaperone)

Filch_22
(tail assembly chaperone)

Filch_23
(tape measure protein)

Filch_24
(minor tail protein)

Filch_25
(minor tail protein)

Filch_26
(minor tail protein)

Filch_27
(minor tail protein)

Filch_28
(minor tail protein, D-ala-D-ala carboxypeptidase)

Filch_29
(minor tail protein)

Filch_30

Filch_31
(helix-turn-helix DNA binding domain)

Filch_32

Filch_33
(lysin A)

Filch_34
(holin)

Filch_35

Filch_36
(lysin B)

Filch_37

Filch_38
(Nrdh-like glutaredoxin)

Filch_39

Filch_40

Filch_41
(Lsr2-like DNA bridging protein)

Filch_42

Filch_43

Filch_44

Filch_45

Filch_46

Filch_47

Filch_48

Filch_49

Filch_50

Filch_51

Filch_52

Filch_53
(tyrosine integrase)

Filch_54

Filch_55

Filch_56
(immunity repressor)

Filch_57

Filch_58

Filch_59
(pentapeptide repeat protein)

Filch_60

Filch_61

Filch_62

Filch_63

Filch_64

Filch_65

Filch_66

Filch_67

Filch_68
(exonuclease)

Filch_69

Filch_70
(ssDNA binding protein, ERF family)

Filch_71

Filch_72

Filch_73

Filch_74

Filch_75

Filch_76

Filch_77
(DprA-like DNA processing chain A)

Filch_78
(HNH endonuclease)

Filch_79

Filch_80

Filch_81

Filch_82
(DNAB-like dsDNA helicase)

Filch_83

Filch_84
(WhiB family transcription factor)

Filch_85

Filch_86

Filch_87
(Holliday junction resolvase)

Filch_88

Filch_89
(polynucleotide kinase)

Filch_90

Filch_91

Filch_92

Filch_93
(RNA ligase)

Filch_94
(helix-turn-helix DNA binding domain)

Filch_95
(WhiB family transcription factor)

Filch_96

Filch_97

Filch_98

Filch_99

Filch_100

Filch_101
(DNA methylase)

Filch_102
(ssDNA binding protein)

Filch_103
(ClpP-like protease)

Filch_104

Filch_105

Filch_106
(helix-turn-helix DNA binding protein)

Filch_107
(HNH endonuclease)

Filch_108

Filch_111

Filch_112

Filch_113
(HNH Endonuclease)

Filch_114
(nucleoside deoxyribosyltransferase)

Filch_115

Filch_116
(DnaQ-like DNA polymerase III subunit)

Filch_117

Filch_118
(RecA-like DNA recombinase)

Filch_119

Filch_120

Filch_121

Filch_122

Filch_123

Filch_124

Filch_125

Filch_126

Filch_127

Filch_128

Filch_129

Filch_130
(helix-turn-helix DNA binding domain)

Filch_131

Filch_132

Filch_133

Filch_134

Filch_135

Filch_136

Filch_137

Filch_138

Filch_139

Filch_140

Filch_141

Filch_142

Filch_143

Filch_144

Filch_145

Filch_146

Filch_147

Filch_148

Filch_149

Filch_150

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMZ622163
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 7
Pitt Freezer Box Grid# E12
Available Files
Plaque PictureDownload
GenBank File for PhameratorDownload