Mycobacterium phage Filch
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Detailed Information for Phage Filch
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByPhilip Versluis
Year Found2014
Location FoundIL
Finding InstitutionHope College
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?No
Isolation TemperatureNot entered
GPS Coordinates42.266319 N, 88.024447 W Map
Naming NotesThe name Filch was given because none of the environmental samples that had been personally collected produced an isolated phage. An extra plate from a colleague that yielded plaques was "filched" with permission and purification proceeded.
Filch is also the nickname given to a past dog.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedAug 4, 2020
Sequencing FacilityHope College
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage4944
Genome length (bp)75692
Character of genome ends3' Sticky Overhang
Overhang Length9 bases
Overhang SequenceCGCTTGTCA
GC Content62.9%
Fasta file available?Yes: Download fasta file
Characterization
ClusterE
Subcluster--
Cluster Life CycleTemperate
Lysogeny NotesFilch is a temperate phage, although the plaque morphology produced is mostly clear; Filch is capable of producing a stable lysogen.
Other Cluster Members
Annotating InstitutionUniversity of the Sciences in Philadelphia
Annotation StatusBeing Annotated (Expected completion by 12/18/2020)
Plaque NotesFilch was isolated, and purified at 32 degrees Celsius. After 24 hours Filch exhibited clear plaques with diameter just below one millimeter. Filch continued to grow until 72 hours when it attained a plaque 3-4 millimeters in diameter.
After 48 hours of incubation, a turbid half ring appeared around the clear plaque.

Filch was incubated at 42 and 32 degrees Celsius. Plaques grew at 32 as well as 42, though Filch may be sensitive to the higher temperature.
Has been Phamerated?Yes
Gene List

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Publication Info
Uploaded to GenBank?No
GenBank AccessionNone yet
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 7
Pitt Freezer Box Grid# E12
Available Files
Plaque PictureDownload