Mycobacterium phage SophKB
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Detailed Information for Phage SophKB
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found BySophia McGonagle and Kailey Leclerc
Year Found2022
Location FoundMA United States of America
Finding InstitutionMerrimack College
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation TemperatureNot entered
GPS Coordinates42.14549 N, 72.45588 W Map
Discovery NotesThe location where the soil was collected is a vegetable garden, which grows tomatoes, squash, green beans, and peas. The soil was initially disturbed by a shovel. After four passes with the shovel, sampling was started. The hole dug to collect the sample was approximately 3-4 inches deep. The soil was sold and compact, as it had just rained. The shovel was used to loosen the soil. The tube given in class was used as a vessel to directly scoop up approximately 20mL of soil. The tube was then labeled with the names of the group members, the time that it was collected (6:00 pm) and the date (September 18, 2022). The ambient temperature for the day was 83°F and the relative humidity
was 45%. The soil was collected one day before the isolation was started and was refrigerated for 20 hours before isolation began.
Naming NotesNamed after the students who discovered the phage.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedDec 15, 2022
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage467
Genome length (bp)75585
Character of genome ends3' Sticky Overhang
Overhang Length9 bases
Overhang SequenceCGCTTGTCA
GC Content63.0%
Sequencing NotesThe sequencing reads showed some contamination of the sample by other phages. One of the other phages (present in ~15% of the total reads) was MAckerman. There was also a novel Cluster A1 phage (~7%) which could be partially assembled.
Fasta file available?Yes: Download fasta file
Characterization
ClusterE
Subcluster--
Cluster Life CycleTemperate
Other Cluster Members
Annotating InstitutionMerrimack College
Annotation StatusBeing Annotated (Expected completion by 5/1/2023)
Plaque NotesThe plaques were very clear, but seem to be confluent. The plaques were about 0.1 cm in diameter.
Has been Phamerated?Yes
Gene List

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Publication Info
Uploaded to GenBank?No
GenBank AccessionNone yet
Refseq NumberNone yet
Archiving Info
Archiving status Not in Pitt Archives
Available Files
Plaque PictureDownload