Mycobacterium phage BadStone
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Detailed Information for Phage BadStone
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found BySeiji Shaw
Year Found2018
Location FoundProvidence, RI USA
Finding InstitutionBrown University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature17°C
GPS Coordinates41.832034 N, 71.38012 W Map
Discovery NotesSample found in the entrance of Blackstone Park, near fence post bordering a community garden. The soil was moist with morning dew, under woodchips, dry leaves, moss, and weeds.
Naming NotesThis phage was one of two found from this soil sample. This phage had inconclusive enzyme digest results and was deemed much more interesting than his relatively unambiguous brother. Hence, this phage was named BadStone.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 30, 2019
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage916
Genome length (bp)75679
Character of genome ends3' Sticky Overhang
Overhang Length9 bases
Overhang SequenceCGCTTGTCA
GC Content63.1%
Fasta file available?Yes: Download fasta file
Characterization
ClusterE
Subcluster--
Cluster Life CycleTemperate
Other Cluster Members
Annotating InstitutionUniversity of Houston-Downtown
Annotation StatusIn GenBank
Plaque NotesPlaques are small, circular, and turbid in nature, ranging typically from 1-3mm.
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List

Click to View

BadStone_1

BadStone_2

BadStone_3

BadStone_4

BadStone_5
(terminase small subunit)

BadStone_6

BadStone_7

BadStone_8
(terminase large subunit)

BadStone_9

BadStone_10
(portal protein)

BadStone_11
(capsid maturation protease)

BadStone_12
(major capsid protein)

BadStone_13

BadStone_14
(head-to-tail adaptor)

BadStone_15
(head-to-tail stopper)

BadStone_16

BadStone_17
(tail terminator)

BadStone_18
(major tail protein)

BadStone_19
(tail assembly chaperone)

BadStone_20
(tail assembly chaperone)

BadStone_21
(tape measure protein)

BadStone_22
(minor tail protein)

BadStone_23
(minor tail protein)

BadStone_24
(minor tail protein)

BadStone_25
(minor tail protein)

BadStone_26
(minor tail protein)

BadStone_27

BadStone_28

BadStone_29
(helix-turn-helix DNA binding domain protein)

BadStone_30

BadStone_31
(lysin A)

BadStone_32
(holin)

BadStone_33

BadStone_34
(lysin B)

BadStone_35

BadStone_36
(NrdH-like glutaredoxin)

BadStone_37
(HNH endonuclease)

BadStone_38

BadStone_39
(Lsr2-like DNA bridging protein)

BadStone_40

BadStone_41

BadStone_42

BadStone_43

BadStone_44

BadStone_45

BadStone_46

BadStone_47

BadStone_48

BadStone_49

BadStone_50
(tyrosine integrase)

BadStone_51

BadStone_52

BadStone_53
(immunity repressor)

BadStone_54

BadStone_55

BadStone_56
(pentapeptide repeat protein)

BadStone_57

BadStone_58

BadStone_59

BadStone_60

BadStone_61

BadStone_62

BadStone_63

BadStone_64
(exonuclease)

BadStone_65

BadStone_66
(ERF family ssDNA binding protein)

BadStone_67

BadStone_68

BadStone_69

BadStone_70

BadStone_71

BadStone_72

BadStone_73
(DprA-like DNA processing chain A)

BadStone_74
(HNH endonuclease)

BadStone_75

BadStone_76

BadStone_77
(DnaB-like dsDNA helicase)

BadStone_78
(DnaB-like dsDNA helicase)

BadStone_79

BadStone_80
(WhiB family transcription factor)

BadStone_81

BadStone_82

BadStone_83
(holliday junction resolvase)

BadStone_84

BadStone_85
(polynucleotide kinase)

BadStone_86

BadStone_87

BadStone_88
(RNA ligase)

BadStone_89
(helix-turn-helix DNA binding domain protein)

BadStone_90
(WhiB family transcription factor)

BadStone_91

BadStone_92

BadStone_93

BadStone_94

BadStone_95

BadStone_96
(DNA methylase)

BadStone_97
(ssDNA binding protein)

BadStone_98
(ClpP-like protease)

BadStone_99

BadStone_100

BadStone_101

BadStone_102
(helix-turn-helix DNA binding domain protein)

BadStone_103
(HNH endonuclease)

BadStone_106

BadStone_107

BadStone_108

BadStone_109

BadStone_110

BadStone_111
(DNA polymerase III subunit)

BadStone_112

BadStone_113

BadStone_114

BadStone_115

BadStone_116
(RecA-like DNA recombinase)

BadStone_117

BadStone_118

BadStone_119

BadStone_120

BadStone_121

BadStone_122

BadStone_123

BadStone_124

BadStone_125

BadStone_126

BadStone_127

BadStone_128
(helix-turn-helix DNA binding domain protein)

BadStone_129
(ParB-like nuclease domain protein)

BadStone_130

BadStone_131

BadStone_132

BadStone_133

BadStone_134

BadStone_135

BadStone_136

BadStone_137

BadStone_138

BadStone_139

BadStone_140

BadStone_141

BadStone_142

BadStone_143

BadStone_144

BadStone_145
(HNH endonuclease)

Submitted Annotation in DNA Master FormatDownload
Submitted Minimal DNA Master FileDownload
Author ListDownload
Annotation Cover SheetDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMT114165
Refseq NumberNone yet
Archiving Info
Archiving status Not in Pitt Archives
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
GenBank File for PhameratorDownload