The Actinobacteriophage Database

Mycobacterium phage HufflyPuff

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Detailed Information for Phage HufflyPuff
Discovery Information
Isolation Host	Mycobacterium smegmatis mc²155
Found By	Prajapati K, Vu C, Wadhwa N, Wood B
Year Found	2011
Location Found	Los Angeles, CA USA
Finding Institution	University of California, Los Angeles
Program	Unavailable
From enriched soil sample?	Yes
Isolation Temperature	Not entered
GPS Coordinates	34.0695 N, 118.4387 W Map
Discovery Notes	Phage isolation performed by Krishna Prajapati, Christine Vu, Nisha Wadhwa, and Brandi Wood (spring 2011). DNA preparation performed by Krisanavane Reddi. Assembly performed by William Villella (UCLA) and confirmed by Charles Bowman (University of Pittsburgh). Preliminary genome annotation performed by Gregory Asatrian, Kevork Khadarian, Cherise Meyerson, and Laya Reddy (winter 2012). Final annotation analysis performed by Debbie Jacobs-Sera in summer 2013 (University of Pittsburgh).
Sequencing Information
Sequencing Complete?	Yes
Date Sequencing Completed	Aug 5, 2013
Sequencing Facility	The UCLA Genotyping and Sequencing Core
Shotgun Sequencing Method	454 Pyrosequencing
Approximate Shotgun Coverage	320
Genome length (bp)	76323
Character of genome ends	3' Sticky Overhang
Overhang Length	9 bases
Overhang Sequence	CGCTTGTCA
GC Content	63.0%
Fasta file available?	Yes: Download fasta file
Characterization
Cluster	E
Subcluster	--
Cluster Life Cycle	Temperate
Other Cluster Members	Click to View 244 ABCat Adnama Amao AmericanBeauty Argent26 Asriel BaboJay BadStone Balomoji Barbarian Bask21 Bench BigBubba BilboSwaggins Bruin Buck BugsBunny Cactus ChosenOne ChotaBhai Cjw1 Command613 Contagion Cookies CrystalP Czyszczon1 Daikon DoctorDiddles DrDrey Dumbo Dusk Easy2Say Elite2014 Elph10 Emmina Eureka Filch FireRed Flypotenuse Gage Gator Gemini Glexan Goku GoldenSpark Goldilocks GooberAzure HanKaySha Harella Henry Highbury Holt Hoonter Hopey HufflyPuff Icee IHOP Inca JeTaime Kanye Kimchi Kostya Lilac Lilizi Lilpickle MadamMonkfish Manda Marshmallow Maxxinista Mindy Miniwave Misfit MISSy Moldemort Mosby MPhalcon Murica Murphy Myrale Nala NelitzaMV Nimrod NoSleep OrionPax Palpatine Paperbeatsrock Pat3 Petra64142 Phaja Pharsalus PhatBacter Phaux Phrux Policronamos Porky Pumpkin Quallification Rakim Rimmer RiverMonster Saints25 Sassay ShamWow ShereKhan Simpliphy SirDuracell SophKB Sotrice96 Stank Stark StellaBean StolenFromERC Tarkin TBrady12 TeardropMSU Teaspoon Terminus Thresher Tomaszewski Toto Traaww1 Tuco Ukulele Wiggin Willez Xandras xkcd YassJohnny Youngblood
Annotating Institution	Unknown or unassigned
Annotation Status	In GenBank
Plaque Notes	Plaque morphology: heterogeneous mix of large, turbid plaques, with smaller, clear plaques Average plaque size: 5-7 mm diameter
Morphotype	Siphoviridae
Number of Genes	146
Number of tRNAs	2
Has been Phamerated?	Yes
Gene List	Click to View HufflyPuff_1 HufflyPuff_2 HufflyPuff_3 HufflyPuff_4 (terminase small subunit) HufflyPuff_5 HufflyPuff_6 HufflyPuff_7 (terminase large subunit) HufflyPuff_8 HufflyPuff_9 (portal protein) HufflyPuff_10 (capsid maturation protease) HufflyPuff_11 (major capsid protein) HufflyPuff_12 HufflyPuff_13 (head-to-tail adaptor) HufflyPuff_14 (head-to-tail stopper) HufflyPuff_15 HufflyPuff_16 (tail terminator) HufflyPuff_17 (major tail protein) HufflyPuff_18 (tail assembly chaperone) HufflyPuff_19 (tail assembly chaperone) HufflyPuff_20 (HNH endonuclease) HufflyPuff_21 (tape measure protein) HufflyPuff_22 (minor tail protein) HufflyPuff_23 (minor tail protein) HufflyPuff_24 (minor tail protein) HufflyPuff_25 (minor tail protein) HufflyPuff_26 (D-Ala-D-Ala carboxypeptidase) HufflyPuff_27 HufflyPuff_28 HufflyPuff_29 HufflyPuff_30 HufflyPuff_31 (lysin A) HufflyPuff_32 (holin) HufflyPuff_33 HufflyPuff_34 (LysB) HufflyPuff_35 HufflyPuff_36 (NrdH) HufflyPuff_37 (lsr2-like protein) HufflyPuff_38 HufflyPuff_39 HufflyPuff_40 HufflyPuff_41 HufflyPuff_42 HufflyPuff_43 HufflyPuff_44 HufflyPuff_45 HufflyPuff_46 HufflyPuff_47 HufflyPuff_48 HufflyPuff_49 HufflyPuff_50 HufflyPuff_51 HufflyPuff_52 HufflyPuff_53 HufflyPuff_54 (tyrosine integrase) HufflyPuff_55 HufflyPuff_56 HufflyPuff_57 HufflyPuff_58 HufflyPuff_59 HufflyPuff_60 (pentapeptide repeat protein) HufflyPuff_61 HufflyPuff_62 HufflyPuff_63 HufflyPuff_64 HufflyPuff_65 HufflyPuff_66 HufflyPuff_67 HufflyPuff_68 HufflyPuff_69 HufflyPuff_70 HufflyPuff_71 HufflyPuff_72 (ssDNA binding protein, ERF family) HufflyPuff_73 HufflyPuff_74 HufflyPuff_75 HufflyPuff_76 HufflyPuff_77 HufflyPuff_78 HufflyPuff_79 HufflyPuff_80 (HNH domain protein) HufflyPuff_81 HufflyPuff_82 HufflyPuff_83 HufflyPuff_84 (DnaB-like helicase) HufflyPuff_85 HufflyPuff_86 (WhiB family transcription factor) HufflyPuff_87 HufflyPuff_88 HufflyPuff_89 HufflyPuff_90 HufflyPuff_91 (polynucleotide kinase) HufflyPuff_92 HufflyPuff_93 (RNA ligase) HufflyPuff_94 HufflyPuff_95 (WhiB) HufflyPuff_96 HufflyPuff_97 HufflyPuff_98 HufflyPuff_99 HufflyPuff_100 HufflyPuff_101 (DNA methylase) HufflyPuff_102 (ssDNA binding protein) HufflyPuff_103 (ClpP-like protease) HufflyPuff_104 HufflyPuff_105 HufflyPuff_106 HufflyPuff_107 HufflyPuff_108 HufflyPuff_111 HufflyPuff_112 HufflyPuff_113 HufflyPuff_114 HufflyPuff_115 (DnaQ-like DNA polymerase III subunit) HufflyPuff_116 HufflyPuff_117 (RecA-like DNA recombinase) HufflyPuff_118 HufflyPuff_119 HufflyPuff_120 HufflyPuff_121 HufflyPuff_122 HufflyPuff_123 HufflyPuff_124 HufflyPuff_125 HufflyPuff_126 HufflyPuff_127 HufflyPuff_128 HufflyPuff_129 HufflyPuff_130 HufflyPuff_131 HufflyPuff_132 HufflyPuff_133 HufflyPuff_134 HufflyPuff_135 HufflyPuff_136 HufflyPuff_137 HufflyPuff_138 HufflyPuff_139 HufflyPuff_140 HufflyPuff_141 HufflyPuff_142 HufflyPuff_143 HufflyPuff_144 HufflyPuff_145 HufflyPuff_146 HufflyPuff_147 HufflyPuff_148 (HNH domain protein)
Publication Info
Uploaded to GenBank?	Yes
GenBank Accession	KF562100
Refseq Number	NC_022981
Archiving Info
Archiving status	Archived
Pitt Freezer Box#	3
Pitt Freezer Box Grid#	E3
Available Files
Plaque Picture	Download
EM Picture	Download
Final DNAMaster File	Download

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