| Detailed Information for Phage Asriel |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Sierra Davis |
| Year Found | 2016 |
| Location Found | Rock Hill, SC USA |
| Finding Institution | Winthrop University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Unknown |
| Isolation Temperature | Not entered |
| GPS Coordinates | 34.9401 N, 81.0316 W Map |
| Discovery Notes | Weather was humid (31.6 degrees Celsius) and soil was dry. |
| Naming Notes | The virus was named after a character from the video game Undertale. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Nov 30, 2016 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 4165 |
| Genome length (bp) | 74594 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 9 bases |
| Overhang Sequence | CGCTTGTCA |
| GC Content | 62.8% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | E |
| Subcluster | -- |
| Cluster Life Cycle | Temperate |
| Lysogeny Notes | -Likely a lytic virus
-TEM Photos reveal siphoviridae shape |
| Other Cluster Members |
|
| Annotating Institution | Unknown or unassigned |
| Annotation Status | In GenBank |
| Plaque Notes | -Round, clear plaques
-About 2 mm in diameter
-Phage heads were 74.146 nm in diameter on average
-Phage tails were about 278.1762 nm in ln on average |
| Morphotype | Siphoviridae |
| Number of Genes | 142 |
| Number of tRNAs | 2 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MG872831 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
2.25 * 10^8 |
| Date of SEA Lysate Titering |
Oct 13, 2016 |
| Pitt Freezer Box# |
43 |
| Pitt Freezer Box Grid# |
A11 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| Final DNAMaster File | Download |
| GenBank File for Phamerator | Download |
