Mycobacterium phage Asriel
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Detailed Information for Phage Asriel
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found BySierra Davis
Year Found2016
Location FoundRock Hill, SC USA
Finding InstitutionWinthrop University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Unknown
Isolation TemperatureNot entered
GPS Coordinates34.9401 N, 81.0316 W Map
Discovery NotesWeather was humid (31.6 degrees Celsius) and soil was dry.
Naming NotesThe virus was named after a character from the video game Undertale.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedNov 30, 2016
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina
Sequencer UsedIllumina MiSeq
Read TypeSingle-end reads
Read Length150 bp
Approximate Shotgun Coverage4165
Genome length (bp)74594
Character of genome ends3' Sticky Overhang
Overhang Length9 bases
Overhang SequenceCGCTTGTCA
GC Content62.8%
Fasta file available?Yes: Download fasta file
Characterization
ClusterE
Subcluster--
Cluster Life CycleTemperate
Lysogeny Notes-Likely a lytic virus
-TEM Photos reveal siphoviridae shape
Other Cluster Members
Annotating InstitutionUnknown or unassigned
Annotation StatusIn GenBank
Plaque Notes-Round, clear plaques
-About 2 mm in diameter
-Phage heads were 74.146 nm in diameter on average
-Phage tails were about 278.1762 nm in ln on average
MorphotypeSiphoviridae
Number of Genes142
Number of tRNAs2
Number of tmRNAs0
Has been Phamerated?Yes
Gene List
Submitted Annotation in DNA Master FormatDownload
Submitted Minimal DNA Master FileDownload
Author ListDownload
Annotation Cover SheetDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMG872831
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Lysate Titer 2.25 * 10^8
Date of SEA Lysate Titering Oct 13, 2016
Pitt Freezer Box# 43
Pitt Freezer Box Grid# A11
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
Final DNAMaster FileDownload
GenBank File for PhameratorDownload