| Detailed Information for Phage BadStone |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Seiji Shaw |
| Year Found | 2018 |
| Location Found | Providence, RI USA |
| Finding Institution | Brown University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 17°C |
| GPS Coordinates | 41.832034 N, 71.38012 W Map |
| Discovery Notes | Sample found in the entrance of Blackstone Park, near fence post bordering a community garden. The soil was moist with morning dew, under woodchips, dry leaves, moss, and weeds. |
| Naming Notes | This phage was one of two found from this soil sample. This phage had inconclusive enzyme digest results and was deemed much more interesting than his relatively unambiguous brother. Hence, this phage was named BadStone. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 30, 2019 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 916 |
| Genome length (bp) | 75679 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 9 bases |
| Overhang Sequence | CGCTTGTCA |
| GC Content | 63.1% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | E |
| Subcluster | -- |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | University of Houston-Downtown |
| Annotation Status | In GenBank |
| Plaque Notes | Plaques are small, circular, and turbid in nature, ranging typically from 1-3mm. |
| Morphotype | Siphoviridae |
| Number of Genes | 143 |
| Number of tRNAs | 2 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MT114165 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Not in Pitt Archives |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| GenBank File for Phamerator | Download |
