| Detailed Information for Phage Barbarian |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Vikrant Dhindsa |
| Year Found | 2016 |
| Location Found | Blacksburg, VA USA |
| Finding Institution | Virginia Tech |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 37.216905 N, 80.449567 W Map |
| Discovery Notes | This phage was found in Foxridge Apartments in soil. The air temperature was 28C and it was found at a depth of 7cm. The soil was fairly moist
and the site was surrounded by flowers. Lastly, the soil seemed new. |
| Naming Notes | I named my phage Barbarian because he is a very strong and savage-like phage, he created many, small plaques every time he infected the M. smegmatis. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 5, 2017 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 682 |
| Genome length (bp) | 74594 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 9 bases |
| Overhang Sequence | CGCTTGTCA |
| GC Content | 62.8% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | E |
| Subcluster | -- |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | Unknown or unassigned |
| Annotation Status | In GenBank |
| Plaque Notes | Temperate |
| Morphotype | Siphoviridae |
| Number of Genes | 144 |
| Number of tRNAs | 2 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MG872832 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
7.7 x 10^7 pfu/mL |
| Pitt Freezer Box# |
31 |
| Pitt Freezer Box Grid# |
B2 |
| Available Files |
| EM Picture | Download |
| Final DNAMaster File | Download |
| GenBank File for Phamerator | Download |