| Detailed Information for Phage Bench |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Zach Romero |
| Year Found | 2021 |
| Location Found | Bowling Green, KY United States |
| Finding Institution | Western Kentucky University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 30°C |
| GPS Coordinates | 36.986333 N, 86.454611 W Map |
| Discovery Notes | Location: in front of McLean Hall Sign on WKU Campus
Soil: Damp; included organic matter such as sticks and beads/rocks
Depth: 2 inches below the soil
Ambient Temperature: 27.7 degrees Celsius |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 21, 2022 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 1438 |
| Genome length (bp) | 76092 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 9 bases |
| Overhang Sequence | CGCTTGTCA |
| GC Content | 63.1% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | E |
| Subcluster | -- |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | Western Kentucky University |
| Annotation Status | In GenBank |
| Plaque Notes | Plaques can be categorized as "bullseye." Larger plaques have significant halos around them, while smaller plaques are less distinct. The bullseye plaques indicate a lytic phage that has not fully lysed a cell, or is less efficient at during lysis. Most of the plaque shows clearness, while the outside of the cell indicates turbidity. |
| Morphotype | Siphoviridae |
| Number of Genes | 147 |
| Number of tRNAs | 2 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | ON456345 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
2.24 x 10^10 pfu/ml |
| Pitt Freezer Box# |
137 |
| Pitt Freezer Box Grid# |
G3 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
