| Detailed Information for Phage Budgie | |
| Discovery Information | |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Cecelia Amorim |
| Year Found | 2025 |
| Location Found | Bowling Green, KY United States |
| Finding Institution | Western Kentucky University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 30°C |
| GPS Coordinates | 37.12876 N, 86.35605 W Map |
| Discovery Notes | Location: Soil taken from a pepper garden bed at Culp Farm in Bowling Green, Kentucky. Soil Conditions: Dry and cracking on the surface, moist under the surface. The soil was collected a few centimeters deep, so it was undisturbed. Temperature: 74°F or 23.3°C. The weather was sunny and mild. |
| Naming Notes | Named after a small bird species. |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 15, 2026 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina NextSeq 1000 |
| Read Type | Single-end reads |
| Read Length | 100 bp |
| Approximate Shotgun Coverage | 510 |
| Genome length (bp) | 76019 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 9 bases |
| Overhang Sequence | CGCTTGTCA |
| GC Content | 62.7% |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | E |
| Subcluster | -- |
| Cluster Life Cycle | Temperate |
| Lysogeny Notes | The lysogeny of the phage Budgie most closely resembles a temperate phage, as some cells were lysed and others lysogenized in the center of the plaques. This pattern of lysogeny (both lytic and lysogenic) is characteristic of temperate phages. This lysogeny pattern could also be due to mutations in the phage genome affecting the lytic cycle, as it is a result that has been repeatedly observed across multiple plaque assays. |
| Other Cluster Members |
Click to View244 ABCat Adnama Amao AmericanBeauty Argent26 Asriel BaboJay BadStone Balomoji Barbarian Bask21 Becksu Bench BigBubba BilboSwaggins Bogg Brewer Bruin Buck Budgie BugsBunny Cactus ChosenOne ChotaBhai Cjw1 Command613 Contagion Cookies CrystalP Czyszczon1 Daikon DoctorDiddles DrDrey Dumbo Dusk Easy2Say Elite2014 Elph10 Emmina Eureka FairyPath Filch FireRed Flypotenuse FoghornLeghorn Gage Gator Gemini Glexan Goku GoldenSpark Goldilocks GooberAzure HanKaySha Harella Henry Highbury Holt Hoonter Hopey HufflyPuff Icee IHOP Inca JeTaime Kanye Kimchi Kostya Lilac Lilizi Lilpickle MadamMonkfish Manda Marshmallow Maxxinista Mindy Miniwave Misfit MISSy Moldemort MollyToe Mosby MPhalcon Murica Murphy Myrale Nala NelitzaMV Nimrod NonchalantPear NoSleep OrionPax Palpatine Paperbeatsrock Pat3 Petra64142 Phaja Pharsalus PhatBacter Phaux Phrux Policronamos Porky Pumpkin Quallification Rakim Rimmer RiverMonster Saints25 Sassay ShamWow ShereKhan Simpliphy SirDuracell SophKB Sotrice96 Stank Stark StellaBean StolenFromERC StormChicken Tarkin TBrady12 TeamEdward TeardropMSU Teaspoon Terminus Thimble Thresher Tomaszewski Toto Traaww1 Tuco Ukulele Wiggin Willez Xandras xkcd YassJohnny Youngblood |
| Annotating Institution | Western Kentucky University |
| Annotation Status | Being Annotated (Expected completion by 5/1/2026) |
| Plaque Notes | There are a mix of both clear, lytic plaques and cloudy, lysogenic plaques. The lysogenic plaques have bullseye patterns or rings in the center of the plaque. Plaque size varies among both lytic and lysogenic plaques. The largest plaques measure 0.2cm, and the smallest plaques measure 0.1cm. The mean of plaque sizes is 0.16cm. The mode and median is 0.2cm. |
| Morphotype | Siphoviridae |
| Number of Genes | 150 |
| Number of tRNAs | 1 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
Click to ViewBudgie_1 Budgie_2 Budgie_3 Budgie_4 Budgie_5 Budgie_6 Budgie_7 Budgie_8 Budgie_9 Budgie_10 Budgie_11 Budgie_12 Budgie_13 Budgie_14 Budgie_15 Budgie_16 Budgie_17 Budgie_18 Budgie_19 Budgie_20 Budgie_21 Budgie_22 Budgie_23 Budgie_24 Budgie_25 Budgie_26 Budgie_27 Budgie_28 Budgie_29 Budgie_30 Budgie_31 Budgie_32 Budgie_33 Budgie_34 Budgie_35 Budgie_36 Budgie_37 Budgie_38 Budgie_39 Budgie_40 Budgie_41 Budgie_42 Budgie_43 Budgie_44 Budgie_45 Budgie_46 Budgie_47 Budgie_48 Budgie_49 Budgie_50 Budgie_51 Budgie_52 Budgie_53 Budgie_54 Budgie_55 Budgie_56 Budgie_57 Budgie_58 Budgie_59 Budgie_60 Budgie_61 Budgie_62 Budgie_63 Budgie_64 Budgie_65 Budgie_66 Budgie_67 Budgie_68 Budgie_69 Budgie_70 Budgie_71 Budgie_72 Budgie_73 Budgie_74 Budgie_75 Budgie_76 Budgie_77 Budgie_78 Budgie_79 Budgie_80 Budgie_81 Budgie_82 Budgie_83 Budgie_84 Budgie_85 Budgie_86 Budgie_87 Budgie_88 Budgie_89 Budgie_90 Budgie_91 Budgie_92 Budgie_93 Budgie_94 Budgie_95 Budgie_96 Budgie_97 Budgie_98 Budgie_99 Budgie_100 Budgie_101 Budgie_102 Budgie_103 Budgie_104 Budgie_105 Budgie_106 Budgie_107 Budgie_108 Budgie_109 Budgie_110 Budgie_111 Budgie_112 Budgie_113 Budgie_114 Budgie_115 Budgie_116 — tRNA Budgie_117 Budgie_118 Budgie_119 Budgie_120 Budgie_121 Budgie_122 Budgie_123 Budgie_124 Budgie_125 Budgie_126 Budgie_127 Budgie_128 Budgie_129 Budgie_130 Budgie_131 Budgie_132 Budgie_133 Budgie_134 Budgie_135 Budgie_136 Budgie_137 Budgie_138 Budgie_139 Budgie_140 Budgie_141 Budgie_142 Budgie_143 Budgie_144 Budgie_145 Budgie_146 Budgie_147 Budgie_148 Budgie_149 Budgie_150 Budgie_151 |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Not in Pitt Archives |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| Virtual Digest Picture | Download |
| EM Picture | Download |
