Mycobacterium phage Budgie
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| Detailed Information for Phage Budgie | |
| Discovery Information | |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Cecelia Amorim |
| Year Found | 2025 |
| Location Found | Bowling Green, KY United States |
| Finding Institution | Western Kentucky University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 30°C |
| GPS Coordinates | 37.12876 N, 86.35605 W Map |
| Discovery Notes | Location: Soil taken from a pepper garden bed at Culp Farm in Bowling Green, Kentucky. Soil Conditions: Dry and cracking on the surface, moist under the surface. The soil was collected a few inches deep, so it was undisturbed. Temperature: 74°F or 23.3°C. The weather was sunny and mild. |
| Naming Notes | Named after a small bird species. |
| Sequencing Information | |
| Sequencing Complete? | No |
| Genome length (bp) | Unknown |
| Character of genome ends | Unknown |
| Fasta file available? | No |
| Characterization | |
| Cluster | Unclustered |
| Subcluster | -- |
| Lysogeny Notes | The lysogeny of the phage Budgie most closely resembles a temperate phage, as some cells were lysed and others lysogenized in the center of the plaques. This pattern of lysogeny (both lytic and lysogenic) is characteristic of temperate phages. This lysogeny pattern could also be due to mutations in the phage genome affecting the lytic cycle, as it is a result that has been repeatedly observed across multiple plaque assays. |
| Annotating Institution | Unknown or unassigned |
| Annotation Status | Not sequenced |
| Plaque Notes | There are a mix of both clear, lytic plaques and cloudy, lysogenic plaques. The lysogenic plaques have bullseye patterns or rings in the center of the plaque. Plaque size varies among both lytic and lysogenic plaques. The largest plaques measure 0.2cm, and the smallest plaques measure 0.1cm. The mean of plaque sizes is 0.16cm. The mode and median is 0.2cm. |
| Has been Phamerated? | No |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Not in Pitt Archives |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
