| Detailed Information for Phage Cactus |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Jalen Smith |
| Year Found | 2011 |
| Location Found | Atlanta, GA USA |
| Finding Institution | Morehouse College |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 33.748326 N, 84.414507 W Map |
| Discovery Notes | saturated soil, 1 inch depth, 86 Fahrenheit, The soil was found in a shady area. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Feb 3, 2012 |
| Genome length (bp) | 75603 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 9 bases |
| Overhang Sequence | CGCTTGTCA |
| GC Content | 63.0% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | E |
| Subcluster | -- |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | Purdue University |
| Annotation Status | In GenBank |
| Plaque Notes | 1942 micrometers when measured. The plaque is clear. |
| Morphotype | Siphoviridae |
| Number of Genes | 152 |
| Number of tRNAs | 2 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MT723943 |
| Refseq Number | None yet |
| Published in Paper | Pope, Bowman, Russell, et al 2015, eLIFE: Whole genome comparison of a large collection of mycobacteriophages reveals a continuum of phage genetic diversity |
| Archiving Info |
| Archiving status |
Archived |
| SEA Designator |
2011MOREcactusJTS |
| SEA Lysate Titer |
5.52e10 |
| Date of SEA Lysate Titering |
Nov 30, 2011 |
| Pitt Freezer Box# |
26 |
| Pitt Freezer Box Grid# |
58 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
