| Detailed Information for Phage ChotaBhai |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Anas Gondal |
| Year Found | 2017 |
| Location Found | Bowling Green, KY USA |
| Finding Institution | Western Kentucky University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 30°C |
| GPS Coordinates | 36.98694 N, 86.45416 W Map |
| Discovery Notes | Soil sample obtained outside of 1509 College Heights Blvd. on August 28th 2017. Humidity was 92% and pressure was 30 inHg. Winds were 4 mph, temperature was 70 degrees Fahrenheit. Sample was obtained at 10:02 P.M. |
| Naming Notes | The name means "Little Brother" in Urdu. The phages name can be paired with the name of the phage BaraBhai, which means big brother in Urdu |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Apr 13, 2018 |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 197 |
| Genome length (bp) | 76145 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 9 bases |
| Overhang Sequence | CGCTTGTCA |
| GC Content | 62.9% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | E |
| Subcluster | -- |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | Western Kentucky University |
| Annotation Status | In GenBank |
| Plaque Notes | Plaques were approximately 1 mm in width and lytic. Plaques were circular in shape and had a smooth border |
| Morphotype | Siphoviridae |
| Number of Genes | 148 |
| Number of tRNAs | 2 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MK801726 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
51 |
| Pitt Freezer Box Grid# |
G3 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
