Mycobacterium phage Cookies
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Detailed Information for Phage Cookies
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByJoshua Beal, Cathleen, Jessica, Austin
Year Found2014
Location FoundReno, NV USA
Finding InstitutionTruckee Meadows Community College
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Unknown
Isolation Temperature37°C
GPS Coordinates38.917222 N, 119.731111 W Map
Discovery NotesSample taken from a raised garden of amended soil collected 9-17-2014
Naming NotesThis phage was originally named Battleborn and then Big John. The phage name was changed in memory of Joshua Beal who passed away shortly after purification. Cookies was chosen by Joshua's wife and it was a favorite word of his.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 4, 2016
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina
Approximate Shotgun Coverage98
Genome length (bp)76340
Character of genome ends3' Sticky Overhang
Overhang Length9 bases
Overhang SequenceCGCTTGTCA
GC Content63.0%
Fasta file available?Yes: Download fasta file
Characterization
ClusterE
Subcluster--
Cluster Life CycleTemperate
Other Cluster Members
Annotating InstitutionTruckee Meadows Community College
Annotation StatusIn GenBank
MorphotypeSiphoviridae
Number of Genes146
Number of tRNAs2
Number of tmRNAs0
Has been Phamerated?Yes
Gene List

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(terminase small subunit)

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(terminase large subunit)

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(portal protein)

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(capsid maturation protease)

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(major capsid protein)

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(head-to-tail adaptor)

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(head-to-tail stopper)

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(tail terminator)

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(major tail protein)

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(tail assembly chaperone)

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(tail assembly chaperone)

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(tape measure protein)

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(minor tail protein)

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(minor tail protein)

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(minor tail protein)

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(minor tail protein)

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(D-ala-D-ala carboxypeptidase)

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(lysin A)

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(holin)

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(lysin B)

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(NrdH-like glutaredoxin)

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(Lsr2-like DNA bridging protein)

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(tyrosine integrase)

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(helix-turn-helix binding protein)

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(exonuclease)

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(ERF family ssDNA binding protein)

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(DNA binding protein)

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(HNH endonuclease)

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(dsDNA helicase)

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(WhiB family transcription factor)

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(holliday junction resolvase)

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(polynucleotide kinase)

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(RNA ligase)

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(WhiB family transcription factor)

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(DNA methylase)

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(ssDNA binding protein)

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(ClpP-like protease)

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Cookies_104 — tRNA
(tRNA-Arg (cct))

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(tRNA-Gly (tcc))

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(DnaQ-like DNA polymerase III subunit)

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(RecA-like DNA recombinase)

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(DNA binding protein)

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Submitted Annotation in DNA Master FormatDownload
Submitted Minimal DNA Master FileDownload
Author ListDownload
Annotation Cover SheetDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMK359320
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 87
Pitt Freezer Box Grid# E8
Available Files
Restriction Digest PictureDownload
EM PictureDownload
Final DNAMaster FileDownload
GenBank File for PhameratorDownload