| Detailed Information for Phage Czyszczon1 |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Emilia Czyszczon |
| Year Found | 2010 |
| Location Found | Bedford, IN USA |
| Finding Institution | Purdue University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 38.797861 N, 86.547 W Map |
| Discovery Notes | Location: Bluespring Caverns in Bedford, Indiana
Date: August 29, 2010
Air Temperature: 54°F (12.22°C)
Water Temperature: 54°F (12.22°C)
Soil Depth: 1 cm from top layer
Soil Type: Glacial Mud
Soil Moisture Content: Saturated
Urban or Rural: Rural |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jun 13, 2013 |
| Sequencing Facility | Purdue Genomics Facility |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 260 |
| Genome length (bp) | 75075 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 9 bases |
| Overhang Sequence | CGCTTGTCA |
| GC Content | 62.9% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | E |
| Subcluster | -- |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | Purdue University |
| Annotation Status | In GenBank |
| Plaque Notes | Size: 0.5-1.5 mm radius
Clear, round plaques with a thicker membrane surrounding |
| Morphotype | Siphoviridae |
| Number of Genes | 144 |
| Number of tRNAs | 2 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MK359309 |
| Refseq Number | None yet |
| Published in Paper | Pope, Bowman, Russell, et al 2015, eLIFE: Whole genome comparison of a large collection of mycobacteriophages reveals a continuum of phage genetic diversity |
| Archiving Info |
| Archiving status |
Archived |
| SEA Designator |
2010PURDczyszczon1EAC |
| Pitt Freezer Box# |
16 |
| Pitt Freezer Box Grid# |
11 |
| Available Files |
| Plaque Picture | Download |
| EM Picture | Download |
| Final DNAMaster File | Download |
| GenBank File for Phamerator | Download |
