| Detailed Information for Phage Daikon |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Mina Cho |
| Year Found | 2023 |
| Location Found | Providence, RI USA |
| Finding Institution | Brown University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 41.823833 N, 71.396694 W Map |
| Discovery Notes | Collected at 10:45 am from Brown University Student Gardens. There was a very light rain and it was approximately 24°C. The soil was loosely packed, and the top inch of soil was used for sampling. |
| Naming Notes | Named after a great refreshing snack: pickled Daikon radish. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 23, 2024 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 954 |
| Genome length (bp) | 75667 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 9 bases |
| Overhang Sequence | CGCTTGTCA |
| GC Content | 62.9% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | E |
| Subcluster | -- |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | Brown University |
| Annotation Status | Overdue (Expected completion by 5/1/2024) |
| Plaque Notes | Despite undergoing 5+ rounds of serial dilution purification, Daikon exhibits two distinct different plaque types: clear and turbid. In each purification round, an isolated turbid, "halo", plaque was chosen for further use, but in subsequent plates, clear plaques still appeared. |
| Morphotype | Siphoviridae |
| Number of Genes | 146 |
| Number of tRNAs | 2 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Publication Info |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Not in Pitt Archives |