| Detailed Information for Phage DoctorDiddles |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Connor Patterson |
| Year Found | 2017 |
| Location Found | Bowling Green, KY USA |
| Finding Institution | Western Kentucky University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Unknown |
| Isolation Temperature | 27°C |
| GPS Coordinates | 37.0021 N, 86.4188 W Map |
| Discovery Notes | The phage was discovered in the soil on the shore of a creek, it was the soil at the surface. It was a very warm day when discovered, 80 degrees Fahrenheit with minimal clouds at 12:18 PM. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Apr 13, 2018 |
| Shotgun Sequencing Method | Illumina |
| Approximate Shotgun Coverage | 200 |
| Genome length (bp) | 75070 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 9 bases |
| Overhang Sequence | CGCTTGTCA |
| GC Content | 62.8% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | E |
| Subcluster | -- |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | Western Kentucky University |
| Annotation Status | In GenBank |
| Plaque Notes | The plaques that formed from this phage were bulls eye shaped. The plaques were non-turbid and very clear in the middle of the plaque but the edges of the plaque were more turbid and unclear. |
| Morphotype | Siphoviridae |
| Number of Genes | 143 |
| Number of tRNAs | 2 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MH399774 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
1.77 * 10^11 pfu |
| Pitt Freezer Box# |
51 |
| Pitt Freezer Box Grid# |
G5 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
