| Detailed Information for Phage Enceladus |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Dani Buchheister & Hannah Sotiroff |
| Year Found | 2020 |
| Location Found | Boulder, CO USA |
| Finding Institution | University of Colorado Boulder |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Unknown |
| Isolation Temperature | -8°C |
| GPS Coordinates | 40.009133 N, 105.269789 W Map |
| Discovery Notes | Found near a classroom building in a landscaped area under a tree. Ground cover was mostly mulch with some bushes and shrubs. |
| Naming Notes | Our phage is named after a giant in Greek mythology. Enceladus is also the name of one of Saturn's icy moons, a promising site for the search for life in the solar system. It's Dani's favorite moon, and it fits since our phage was taken from the soil on a cold and snowy day. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Dec 22, 2020 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 427 |
| Genome length (bp) | 66420 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | TCGAACGGCC |
| GC Content | 58.7% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | L |
| Subcluster | L1 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | University of Colorado Boulder |
| Annotation Status | In GenBank |
| Plaque Notes | Plaques have clear edges and range from 3-5 mm in diameter. |
| Morphotype | Siphoviridae |
| Number of Genes | 109 |
| Number of tRNAs | 9 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MZ958746 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
126 |
| Pitt Freezer Box Grid# |
G4 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
