Detailed Information for Phage FireRed |
Discovery Information |
Isolation Host | Mycobacterium smegmatis mc²155 |
Found By | Optimus Prion |
Year Found | 2013 |
Location Found | Los Angeles, CA USA |
Finding Institution | University of California, Los Angeles |
Program | Unavailable |
From enriched soil sample? | Yes |
Isolation Temperature | 35°C |
GPS Coordinates | 34.08 N, 118.4 W Map |
Discovery Notes | Phage isolation performed in Fall 2013 and genome annotation performed in Spring 2014 by Team Optimus Prion: Joseph Alzagatiti, David Chun, and Benj Jara. Annotation was quality checked by Lei Yeh, Iger Ostreni and Edric Yoon. TA: Khristina Ipapo. Instructor: Jordan Moberg Parker. DNA preparation performed by Krisanavane Reddi. Assembly performed by William Villella (UCLA).
Soil sample collected from a planter adjacent to Shapiro Fountain at UCLA on October 3, 2013. |
Naming Notes | FireRed Version is a Pokemon reference. |
Sequencing Information |
Sequencing Complete? | Yes |
Date Sequencing Completed | Apr 10, 2014 |
Sequencing Facility | The UCLA Genotyping and Sequencing Core |
Shotgun Sequencing Method | 454 Pyrosequencing |
Approximate Shotgun Coverage | 48 |
Genome length (bp) | 76217 |
Character of genome ends | 3' Sticky Overhang |
Overhang Length | 9 bases |
Overhang Sequence | CGCTTGTCA |
GC Content | 63.0% |
Fasta file available? | Yes: Download fasta file |
Characterization |
Cluster | E |
Subcluster | -- |
Cluster Life Cycle | Temperate |
Other Cluster Members |
|
Annotating Institution | Unknown or unassigned |
Annotation Status | In GenBank |
Plaque Notes | Turbid plaques (possibly lysogenic), average diameter of 4mm. |
Morphotype | Siphoviridae |
Number of Genes | 148 |
Number of tRNAs | 2 |
Number of tmRNAs | 0 |
Has been Phamerated? | Yes |
Gene List |
|
Submitted Minimal DNA Master File | Download |
Publication Info |
Uploaded to GenBank? | Yes |
GenBank Accession | MF919506 |
Refseq Number | None yet |
Archiving Info |
Archiving status |
Archived |
Pitt Freezer Box# |
23 |
Pitt Freezer Box Grid# |
C1 |
Available Files |
Plaque Picture | Download |
Restriction Digest Picture | Download |
EM Picture | Download |
GenBank File for Phamerator | Download |