The Actinobacteriophage Database

Mycobacterium phage Gemini

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Detailed Information for Phage Gemini
Discovery Information
Isolation Host	Mycobacterium smegmatis mc²155
Found By	Eric Westerman
Year Found	2010
Location Found	rural, WV USA
Finding Institution	Western Kentucky University
Program	Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?	Yes
Isolation Temperature	Not entered
GPS Coordinates	38.770916 N, 81.31445 W Map
Discovery Notes	The soil type was fertile garden soil. I dug 8.89 centimeters into the soil. The temperature was 68 degrees Fahrenheit. On top of a large hill in West Virginia, near Spenser, WV. I found the sample in a vegetable garden which cantained peppers and tomatoes. Being in the area of a former pasture field and a current residential area, the ground was relatively flat, and the soil must have been tread on often during the gardening process. The garden was surrounded by a fence, but other than that, the closest structure was a driveway, which was approximately 25ft away. The soil was relatively moist, and it should yield some interesting phage do to its fertility.
Sequencing Information
Sequencing Complete?	Yes
Date Sequencing Completed	Mar 1, 2016
Shotgun Sequencing Method	Illumina Sequencing
Approximate Shotgun Coverage	2875
Genome length (bp)	75649
Character of genome ends	3' Sticky Overhang
Overhang Length	9 bases
Overhang Sequence	CGCTTGTCA
GC Content	62.9%
Fasta file available?	Yes: Download fasta file
Characterization
Cluster	E
Subcluster	--
Cluster Life Cycle	Temperate
Other Cluster Members	Click to View 244 ABCat Adnama Amao AmericanBeauty Argent26 Asriel BaboJay BadStone Balomoji Barbarian Bask21 Bench BigBubba BilboSwaggins Bruin Buck BugsBunny Cactus ChosenOne ChotaBhai Cjw1 Command613 Contagion Cookies CrystalP Czyszczon1 Daikon DoctorDiddles DrDrey Dumbo Dusk Easy2Say Elite2014 Elph10 Emmina Eureka Filch FireRed Flypotenuse Gage Gator Gemini Glexan Goku GoldenSpark Goldilocks GooberAzure HanKaySha Harella Henry Highbury Holt Hoonter Hopey HufflyPuff Icee IHOP Inca JeTaime Kanye Kimchi Kostya Lilac Lilizi Lilpickle MadamMonkfish Manda Marshmallow Maxxinista Mindy Miniwave Misfit MISSy Moldemort Mosby MPhalcon Murica Murphy Myrale Nala NelitzaMV Nimrod NoSleep OrionPax Palpatine Paperbeatsrock Pat3 Petra64142 Phaja Pharsalus PhatBacter Phaux Phrux Policronamos Porky Pumpkin Quallification Rakim Rimmer RiverMonster Saints25 Sassay ShamWow ShereKhan Simpliphy SirDuracell SophKB Sotrice96 Stank Stark StellaBean StolenFromERC Tarkin TBrady12 TeardropMSU Teaspoon Terminus Thresher Tomaszewski Toto Traaww1 Tuco Ukulele Wiggin Willez Xandras xkcd YassJohnny Youngblood
Annotating Institution	Western Kentucky University
Annotation Status	In GenBank
Plaque Notes	0.4cm clear plaques with turbid haloes, and also 0.1cm plaques that look clear at first glance, but on closer inspection also have thinner haloes. Of particular note is the unwillingness of plaques to overtake each other, usually forming around instead of over other plaques. Best seen in relationship between big halo and small plaques. Forms half moon shape. 24 hour incubation period in 37°C conditions.
Morphotype	Siphoviridae
Number of Genes	149
Number of tRNAs	2
Number of tmRNAs	0
Has been Phamerated?	Yes
Gene List	Click to View Gemini_1 Gemini_2 Gemini_3 Gemini_4 Gemini_5 Gemini_6 (terminase small subunit) Gemini_7 Gemini_8 Gemini_9 Gemini_10 (terminase large subunit) Gemini_11 Gemini_12 (portal protein) Gemini_13 (capsid maturation protease) Gemini_14 (major capsid protein) Gemini_15 Gemini_16 (head-to-tail adaptor) Gemini_17 (head-to-tail stopper) Gemini_18 (head-to-tail connector complex protein) Gemini_19 (tail terminator) Gemini_20 (major tail protein) Gemini_21 Gemini_22 (tail assembly chaperone) Gemini_23 (tail assembly chaperone) Gemini_24 (HNH endonuclease) Gemini_25 (tape measure protein) Gemini_26 (minor tail protein) Gemini_27 (minor tail protein) Gemini_28 (minor tail protein) Gemini_29 (minor tail protein) Gemini_30 (D-Ala-D-Ala carboxypeptidase) Gemini_31 (minor tail protein) Gemini_32 Gemini_33 (helix-turn-helix DNA-binding domain protein) Gemini_34 Gemini_35 (lysin A) Gemini_36 (holin) Gemini_37 Gemini_38 (lysin B) Gemini_39 Gemini_40 (NrdH-like glutaredoxin) Gemini_41 (Lsr2-like DNA bridging protein) Gemini_42 Gemini_43 Gemini_44 Gemini_45 Gemini_46 Gemini_47 Gemini_48 Gemini_49 Gemini_50 Gemini_51 Gemini_52 Gemini_53 Gemini_54 Gemini_55 Gemini_56 (tyrosine integrase) Gemini_57 Gemini_58 Gemini_59 (immunity repressor) Gemini_60 Gemini_61 Gemini_62 (pentapeptide repeat protein) Gemini_63 Gemini_64 Gemini_65 Gemini_66 Gemini_67 Gemini_68 Gemini_69 Gemini_70 Gemini_71 Gemini_72 (exonuclease) Gemini_73 Gemini_74 (ERF family ssDNA binding protein) Gemini_75 Gemini_76 Gemini_77 Gemini_78 Gemini_79 Gemini_80 Gemini_81 (DprA-like DNA processing chain A) Gemini_82 (HNH endonuclease) Gemini_83 Gemini_84 Gemini_85 Gemini_86 (DnaB-like dsDNA helicase) Gemini_87 Gemini_88 (WhiB family transcription factor) Gemini_89 Gemini_90 Gemini_91 (holliday junction resolvase) Gemini_92 Gemini_93 (polynucleotide kinase) Gemini_94 Gemini_95 Gemini_96 (RNA ligase) Gemini_97 (helix-turn-helix DNA-binding domain protein) Gemini_98 (WhiB family transcription factor) Gemini_99 Gemini_100 Gemini_101 Gemini_102 Gemini_103 Gemini_104 (endonuclease VII) Gemini_105 (ssDNA binding protein) Gemini_106 (ClpP-like protease) Gemini_107 Gemini_108 Gemini_109 Gemini_110 (helix-turn-helix DNA-binding domain protein) Gemini_111 (HNH endonuclease) Gemini_112 — tRNA (codon recognized: AGG) Gemini_113 — tRNA (codon recognized: GGA) Gemini_114 Gemini_115 Gemini_116 Gemini_117 Gemini_118 (DnaQ-like DNA polymerase III subunit) Gemini_119 Gemini_120 (RecA-like DNA recombinase) Gemini_121 Gemini_122 Gemini_123 Gemini_124 Gemini_125 Gemini_126 Gemini_127 Gemini_128 Gemini_129 Gemini_130 Gemini_131 Gemini_132 (helix-turn-helix DNA-binding domain protein) Gemini_133 Gemini_134 Gemini_135 Gemini_136 Gemini_137 Gemini_138 Gemini_139 Gemini_140 Gemini_141 Gemini_142 Gemini_143 Gemini_144 Gemini_145 Gemini_146 Gemini_147 Gemini_148 Gemini_149 Gemini_150 Gemini_151 (HNH endonuclease)
Submitted Minimal DNA Master File	Download
Publication Info
Uploaded to GenBank?	Yes
GenBank Accession	MH779503
Refseq Number	None yet
Archiving Info
Archiving status	Archived
SEA Designator	2010WEKUgeminiEWW
SEA Lysate Titer	7.4e10
Date of SEA Lysate Titering	Oct 5, 2010
Pitt Freezer Box#	20
Pitt Freezer Box Grid#	1
Available Files
Plaque Picture	Download
Restriction Digest Picture	Download
EM Picture	Download
GenBank File for Phamerator	Download

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