Mycobacterium phage Hopey
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Detailed Information for Phage Hopey
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByAllison, Anthony + Miranda
Year Found2013
Location FoundNorth Andover, MA USA
Finding InstitutionMerrimack College
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation TemperatureNot entered
GPS Coordinates42.669331 N, 71.124094 W Map
Discovery NotesFrom a truck tire track on the Merrimack College campus.
Naming NotesOur president, Christopher Hopey.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedDec 10, 2013
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina
Approximate Shotgun Coverage3442
Genome length (bp)75586
Character of genome ends3' Sticky Overhang
Overhang Length9 bases
Overhang SequenceCGCTTGTCA
GC Content63.0%
Fasta file available?Yes: Download fasta file
Characterization
ClusterE
Subcluster--
Cluster Life CycleTemperate
Other Cluster Members
Annotating InstitutionMerrimack College
Annotation StatusIn GenBank
Plaque NotesCloudy with rings around the outer edge
MorphotypeSiphoviridae
Number of Genes142
Number of tRNAs2
Number of tmRNAs0
Has been Phamerated?Yes
Gene List

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Hopey_1

Hopey_2

Hopey_3

Hopey_4

Hopey_5

Hopey_6
(terminase small subunit)

Hopey_7

Hopey_8

Hopey_9
(terminase large subunit)

Hopey_10

Hopey_11
(portal protein)

Hopey_12
(capsid maturation protease)

Hopey_13
(major capsid protein)

Hopey_14

Hopey_15
(head-to-tail adaptor)

Hopey_16
(head-to-tail stopper)

Hopey_17

Hopey_18
(tail terminator)

Hopey_19
(major tail protein)

Hopey_20

Hopey_21
(tail assembly chaperone)

Hopey_22
(tail assembly chaperone)

Hopey_23
(endonuclease VII)

Hopey_24
(tape measure protein)

Hopey_25
(minor tail protein)

Hopey_26
(minor tail protein)

Hopey_27
(minor tail protein)

Hopey_28
(minor tail protein)

Hopey_29
(D-Ala-D-Ala carboxypeptidase)

Hopey_30

Hopey_31

Hopey_32
(helix-turn-helix DNA binding domain protein)

Hopey_33

Hopey_34
(lysin A)

Hopey_35
(holin)

Hopey_36

Hopey_37
(lysin B)

Hopey_38

Hopey_39
(NrdH-like glutaredoxin)

Hopey_40

Hopey_41
(Lsr2-like DNA bridging protein)

Hopey_42

Hopey_43

Hopey_44

Hopey_45

Hopey_46

Hopey_47

Hopey_48

Hopey_49

Hopey_50

Hopey_51

Hopey_52
(tyrosine integrase)

Hopey_53

Hopey_54

Hopey_55
(immunity repressor)

Hopey_56

Hopey_57

Hopey_58
(pentapeptide repeat protein)

Hopey_59

Hopey_60

Hopey_61

Hopey_62

Hopey_63

Hopey_64

Hopey_65

Hopey_66

Hopey_67
(exonuclease)

Hopey_68

Hopey_69
(ERF family ssDNA binding protein)

Hopey_70

Hopey_71

Hopey_72

Hopey_73

Hopey_74

Hopey_75

Hopey_76
(DprA-like DNA processing chain A)

Hopey_77
(HNH endonuclease)

Hopey_78

Hopey_79

Hopey_80

Hopey_81
(DnaB-like dsDNA helicase)

Hopey_82

Hopey_83
(WhiB family transcription factor)

Hopey_84

Hopey_85

Hopey_86
(holliday junction resolvase)

Hopey_87

Hopey_88
(polynucleotide kinase)

Hopey_89

Hopey_90

Hopey_91

Hopey_92
(RNA ligase)

Hopey_93
(helix-turn-helix DNA binding domain protein)

Hopey_94
(WhiB family transcription factor)

Hopey_95

Hopey_96

Hopey_97

Hopey_98

Hopey_99

Hopey_100
(DNA methylase)

Hopey_101
(ssDNA binding protein)

Hopey_102
(ClpP-like protease)

Hopey_103

Hopey_104

Hopey_105
(MerR-like helix-turn-helix DNA binding domain protein)

Hopey_106
(HNH endonuclease)

Hopey_107 — tRNA

Hopey_108 — tRNA

Hopey_109

Hopey_110

Hopey_111
(hydrolase)

Hopey_112

Hopey_113
(DnaQ-like DNA polymerase III subunit)

Hopey_114

Hopey_115
(RecA-like DNA recombinase)

Hopey_116

Hopey_117

Hopey_118

Hopey_119

Hopey_120

Hopey_121

Hopey_122

Hopey_123

Hopey_124

Hopey_125

Hopey_126

Hopey_127
(helix-turn-helix DNA binding domain protein)

Hopey_128

Hopey_129

Hopey_130

Hopey_131

Hopey_132

Hopey_133

Hopey_134

Hopey_135

Hopey_136

Hopey_137

Hopey_138

Hopey_139

Hopey_140

Hopey_141

Hopey_142

Hopey_143

Hopey_144
(HNH endonuclease)

Submitted Annotation in DNA Master FormatDownload
Submitted Minimal DNA Master FileDownload
Author ListDownload
Annotation Cover SheetDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMH576953
Refseq NumberNone yet
Published in PaperPope, Bowman, Russell, et al 2015, eLIFE: Whole genome comparison of a large collection of mycobacteriophages reveals a continuum of phage genetic diversity
Archiving Info
Archiving status Archived
Pitt Freezer Box# 14
Pitt Freezer Box Grid# G9
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload