| Detailed Information for Phage HufflyPuff |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Prajapati K, Vu C, Wadhwa N, Wood B |
| Year Found | 2011 |
| Location Found | Los Angeles, CA USA |
| Finding Institution | University of California, Los Angeles |
| Program | Unavailable |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 34.0695 N, 118.4387 W Map |
| Discovery Notes | Phage isolation performed by Krishna Prajapati, Christine Vu, Nisha Wadhwa, and Brandi Wood (spring 2011). DNA preparation performed by Krisanavane Reddi. Assembly performed by William Villella (UCLA) and confirmed by Charles Bowman (University of Pittsburgh). Preliminary genome annotation performed by Gregory Asatrian, Kevork Khadarian, Cherise Meyerson, and Laya Reddy (winter 2012). Final annotation analysis performed by Debbie Jacobs-Sera in summer 2013 (University of Pittsburgh). |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Aug 5, 2013 |
| Sequencing Facility | The UCLA Genotyping and Sequencing Core |
| Shotgun Sequencing Method | 454 Pyrosequencing |
| Approximate Shotgun Coverage | 320 |
| Genome length (bp) | 76323 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 9 bases |
| Overhang Sequence | CGCTTGTCA |
| GC Content | 63.0% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | E |
| Subcluster | -- |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | Unknown or unassigned |
| Annotation Status | In GenBank |
| Plaque Notes | Plaque morphology: heterogeneous mix of large, turbid plaques, with smaller, clear plaques
Average plaque size: 5-7 mm diameter |
| Morphotype | Siphoviridae |
| Number of Genes | 146 |
| Number of tRNAs | 2 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | KF562100 |
| Refseq Number | NC_022981 |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
3 |
| Pitt Freezer Box Grid# |
E3 |
| Available Files |
| Plaque Picture | Download |
| EM Picture | Download |
| Final DNAMaster File | Download |
