Detailed Information for Phage Icee |
Discovery Information |
Isolation Host | Mycobacterium smegmatis mc²155 |
Found By | Emily Ice |
Year Found | 2016 |
Location Found | Louisville, KY USA |
Finding Institution | Spalding University |
Program | Kentucky Biomedical Infrastructure Network Small Genomes Discovery Program |
From enriched soil sample? | No |
Isolation Temperature | Not entered |
GPS Coordinates | 38.258611 N, 85.748056 W Map |
Discovery Notes | Found by the Ohio River in downtown Louisville. It was a sunny day and the soil was moist. The temperature was 50 degrees Fahrenheit. |
Naming Notes | My last name is unique so I decided to name my phage after it. |
Sequencing Information |
Sequencing Complete? | Yes |
Date Sequencing Completed | Mar 14, 2017 |
Shotgun Sequencing Method | Illumina |
Approximate Shotgun Coverage | 1393 |
Genome length (bp) | 74169 |
Character of genome ends | 3' Sticky Overhang |
Overhang Length | 9 bases |
Overhang Sequence | CGCTTGTCA |
GC Content | 63.0% |
Fasta file available? | Yes: Download fasta file |
Characterization |
Cluster | E |
Subcluster | -- |
Cluster Life Cycle | Temperate |
Other Cluster Members |
|
Annotating Institution | Spalding University |
Annotation Status | In GenBank |
Plaque Notes | Lytic and 1mm in diameter. |
Morphotype | Siphoviridae |
Number of Genes | 144 |
Number of tRNAs | 2 |
Number of tmRNAs | 0 |
Has been Phamerated? | Yes |
Gene List |
|
Submitted Minimal DNA Master File | Download |
Publication Info |
Uploaded to GenBank? | Yes |
GenBank Accession | MH399778 |
Refseq Number | None yet |
Archiving Info |
Archiving status |
Archived |
SEA Lysate Titer |
4.8x10^10 pfu/mL |
Pitt Freezer Box# |
28 |
Pitt Freezer Box Grid# |
B8 |
Available Files |
Plaque Picture | Download |
Restriction Digest Picture | Download |
EM Picture | Download |
GenBank File for Phamerator | Download |