| Detailed Information for Phage Icee |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Emily Ice |
| Year Found | 2016 |
| Location Found | Louisville, KY USA |
| Finding Institution | Spalding University |
| Program | Kentucky Biomedical Infrastructure Network Small Genomes Discovery Program |
| From enriched soil sample? | No |
| Isolation Temperature | Not entered |
| GPS Coordinates | 38.258611 N, 85.748056 W Map |
| Discovery Notes | Found by the Ohio River in downtown Louisville. It was a sunny day and the soil was moist. The temperature was 50 degrees Fahrenheit. |
| Naming Notes | My last name is unique so I decided to name my phage after it. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Mar 14, 2017 |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 1393 |
| Genome length (bp) | 74169 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 9 bases |
| Overhang Sequence | CGCTTGTCA |
| GC Content | 63.0% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | E |
| Subcluster | -- |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | Spalding University |
| Annotation Status | In GenBank |
| Plaque Notes | Lytic and 1mm in diameter. |
| Morphotype | Siphoviridae |
| Number of Genes | 144 |
| Number of tRNAs | 2 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MH399778 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
4.8x10^10 pfu/mL |
| Pitt Freezer Box# |
28 |
| Pitt Freezer Box Grid# |
B8 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
