| Detailed Information for Phage JeTaime |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Chanelle Kendrick |
| Year Found | 2018 |
| Location Found | Providence, RI USA |
| Finding Institution | Providence College |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 37°C |
| GPS Coordinates | 41.84 N, 71.44 W Map |
| Discovery Notes | The temperature outside was 83ºF. It was a sunny days with some clouds. The soil contained a lot of moisture and it was collected near a sprinkler. The sample was accumulated from approximately an inch deep. |
| Naming Notes | The name JeTaime derives from the French phrase " I love you" in French. I am French so I thought it would be interesting to name my phage something connected to the language. I have been working with my phage for a couple months and I have a deep connection with it, so I thought the name fit perfectly. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Aug 31, 2020 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 1389 |
| Genome length (bp) | 75099 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 9 bases |
| Overhang Sequence | CGCTTGTCA |
| GC Content | 62.9% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | E |
| Subcluster | -- |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | College of Charleston |
| Annotation Status | In GenBank |
| Plaque Notes | The individual plaques appear to be clear and circular |
| Morphotype | Siphoviridae |
| Number of Genes | 142 |
| Number of tRNAs | 2 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MZ322024 |
| Refseq Number | None yet |
| Published in Paper | Flota et al 2021, Microbiology Resource Announcements: Evaluation of Genome Sequences of the Bacteriophages JeTaime and Luna22 |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
3.3x10^10 pfu/mL |
| Pitt Freezer Box# |
89 |
| Pitt Freezer Box Grid# |
D7 |
| Available Files |
| Plaque Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
