Mycobacterium phage Lumos
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Detailed Information for Phage Lumos
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByLaura Molina
Year Found2014
Location FoundStuart, FL USA
Finding InstitutionIndian River State College
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature29°C
GPS Coordinates27.176074 N, 80.252566 W Map
Discovery NotesSoil collected from Moore's Cemetery in Stuart, FL. The soil was dry and mixed heavily with sand.
Naming NotesLumos refers to the spell for light in "Harry Potter"
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedDec 10, 2014
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage185
Genome length (bp)75586
Character of genome ends3' Sticky Overhang
Overhang Length10 bases
Overhang SequenceTCGATCAGCC
GC Content59.3%
Fasta file available?Yes: Download fasta file
Characterization
ClusterL
SubclusterL3
Cluster Life CycleTemperate
Other Cluster Members
Annotating InstitutionUnknown or unassigned
Annotation StatusIn GenBank
Plaque NotesClear, all around, approximately 3-4mm in diameter
MorphotypeSiphoviridae
Number of Genes128
Number of tRNAs10
Number of tmRNAs0
Has been Phamerated?Yes
Gene List

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Lumos_1

Lumos_2
(terminase small subunit)

Lumos_3

Lumos_4

Lumos_5
(terminase large subunit)

Lumos_6
(portal protein)

Lumos_7
(MuF-like minor capsid protein)

Lumos_8
(scaffolding protein)

Lumos_9
(major capsid protein)

Lumos_10
(head-to-tail adaptor)

Lumos_11
(head-to-tail stopper)

Lumos_12

Lumos_13
(tail terminator)

Lumos_14
(major tail protein)

Lumos_15
(tail assembly chaperone)

Lumos_16
(tail assembly chaperone)

Lumos_17
(tape measure protein)

Lumos_18
(minor tail protein)

Lumos_19
(minor tail protein)

Lumos_20
(minor tail protein)

Lumos_21

Lumos_22

Lumos_23

Lumos_24

Lumos_25

Lumos_26
(lysin A)

Lumos_27

Lumos_28
(lysin B)

Lumos_29

Lumos_30

Lumos_31
(NrdH-like glutaredoxin)

Lumos_32

Lumos_33
(nucleotidyl transferase)

Lumos_34
(Lsr2-like DNA bridging protein)

Lumos_35

Lumos_36
(tyrosine integrase)

Lumos_37

Lumos_38

Lumos_39
(Cro protein)

Lumos_40

Lumos_41

Lumos_42

Lumos_43

Lumos_44

Lumos_45
(esterase)

Lumos_46

Lumos_47

Lumos_48

Lumos_49
(band-7-like membrane protein)

Lumos_50 — tRNA
(tRNA-Tyr(gta))

Lumos_51

Lumos_52

Lumos_53

Lumos_54

Lumos_55

Lumos_56
(Ro-like RNA binding protein)

Lumos_57

Lumos_58

Lumos_59

Lumos_60

Lumos_61

Lumos_62

Lumos_63
(ssDNA binding protein, ERF family)

Lumos_64

Lumos_65
(ParB-like nuclease domain protein)

Lumos_66

Lumos_67
(HNH endonuclease)

Lumos_68

Lumos_69

Lumos_70
(DnaB-like dsDNA helicase)

Lumos_71
(WhiB family transcription factor)

Lumos_72

Lumos_73

Lumos_74

Lumos_75
(phosphoribosyl pyrophosphate transferase)

Lumos_76
(nicotinate ribosyltransferase)

Lumos_77
(polynucleotide kinase)

Lumos_78

Lumos_79
(RusA-like resolvase)

Lumos_80
(WhiB family transcription factor)

Lumos_81

Lumos_82

Lumos_83

Lumos_84
(ssDNA binding protein)

Lumos_85

Lumos_86
(RNA ligase)

Lumos_87
(ClpP-like protease)

Lumos_88

Lumos_89

Lumos_90

Lumos_91
(excise)

Lumos_92
(HNH endonuclease)

Lumos_93

Lumos_94

Lumos_95
(DnaQ-like DNA polymerase III subunit)

Lumos_96

Lumos_97

Lumos_98
(HNH endonuclease)

Lumos_99

Lumos_100

Lumos_101

Lumos_102

Lumos_103 — tRNA
(tRNA-Thr(cgt))

Lumos_104

Lumos_105 — tRNA
(tRNA-Pro(tgg))

Lumos_106

Lumos_107 — tRNA
(tRNA-Trp(cca))

Lumos_108 — tRNA
(tRNA-Leu(tag))

Lumos_109 — tRNA
(tRNA-His(gtg))

Lumos_110

Lumos_111 — tRNA
(tRNA-Gln(ctg))

Lumos_112 — tRNA
(tRNA-Lys(ctt))

Lumos_113

Lumos_114

Lumos_115 — tRNA
(tRNA-Asn(gtt))

Lumos_116 — tRNA
(tRNA-Lys(ttt))

Lumos_117

Lumos_118

Lumos_119

Lumos_120

Lumos_121

Lumos_122

Lumos_123

Lumos_124

Lumos_125

Lumos_126

Lumos_127

Lumos_128

Lumos_129

Lumos_130

Lumos_131

Lumos_132

Lumos_133

Lumos_134
(AAA-ATPase)

Lumos_135

Lumos_136

Lumos_137

Lumos_138

Submitted Annotation in DNA Master FormatDownload
Submitted Minimal DNA Master FileDownload
Author ListDownload
Annotation Cover SheetDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionKT372003
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Lysate Titer 1.99x10^9
Date of SEA Lysate Titering Oct 22, 2014
Pitt Freezer Box# 10
Pitt Freezer Box Grid# G11