| Detailed Information for Phage MPhalcon |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Team Rage Against the Phage |
| Year Found | 2017 |
| Location Found | Venice, CA USA |
| Finding Institution | University of California, Los Angeles |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | 35°C |
| GPS Coordinates | 33.9922 N, 118.4705 W Map |
| Discovery Notes | Phage MPhalcon was isolated from dark, soft soil by a street-side garden, collected on 10/08/17 by Riley Spurling. Air temp at time of collection was 20.5˚C. MPhalcon was isolated by direct plating at 35C.
Characterization was performed by Team Rage Against the Phage (Riley Spurling, Raveena Mann, Alec Griffith).
Midi-Lysate (Archive) Titer: 1.3e10 pfu/ml
Date of High-titer Lysate Titering: 11/6/16
MIMG103AL-F17 Instructor: Dr. Amanda Freise
Teaching Assistant: Andrew Lund
Undergraduate Assistant: Keith Mitchell
Laboratory Support: Dr. Krisanavane Reddi and Dr. Jordan Moberg Parker |
| Naming Notes | The capsid head of the phage
looks like the Millennium Falcon from Star Wars. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Dec 21, 2017 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 2029 |
| Genome length (bp) | 75605 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 9 bases |
| Overhang Sequence | CGCTTGTCA |
| GC Content | 63.1% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | E |
| Subcluster | -- |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | University of California, Los Angeles |
| Annotation Status | In GenBank |
| Plaque Notes | mix of 2-4mm clear/halo and 2-4mm bullseye. |
| Morphotype | Siphoviridae |
| Number of Genes | 146 |
| Number of tRNAs | 2 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MH020247 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
58 |
| Pitt Freezer Box Grid# |
F4 |
| Available Files |
| Plaque Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
