| Detailed Information for Phage MadamMonkfish |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Natascha Richardson |
| Year Found | 2011 |
| Location Found | Ravenna, KY USA |
| Finding Institution | Western Kentucky University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 37.689 N, 83.946 W Map |
| Discovery Notes | Soil was wet due to recent rainfall. Soil was coarse and contained weed fragments. Sample was taken from a hill about 100 yards from Cow Creek Road in Ravenna, Kentucky. Sample was taken at 11:35 A.M. on September 5th, 2011. Soil was taken from 1-3 inches below the surface. The temperature outside was 19.4 degrees Celsius. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Feb 2, 2012 |
| Sequencing Facility | Virginia Commonwealth University Nucleic Acids Research Facilities |
| Shotgun Sequencing Method | 454 |
| Sequencer Used | 454 Genome Sequencer FLX |
| Approximate Shotgun Coverage | 34 |
| Genome length (bp) | 75554 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 9 bases |
| Overhang Sequence | CGCTTGTCA |
| GC Content | 63.0% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | E |
| Subcluster | -- |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | Unknown or unassigned |
| Annotation Status | In GenBank |
| Plaque Notes | Plaques are small and round with clear centers and a small turbid clearing zone around the plaque. |
| Morphotype | Siphoviridae |
| Number of Genes | 146 |
| Number of tRNAs | 2 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MF668277 |
| Refseq Number | None yet |
| Published in Paper | Pope, Bowman, Russell, et al 2015, eLIFE: Whole genome comparison of a large collection of mycobacteriophages reveals a continuum of phage genetic diversity |
| Archiving Info |
| Archiving status |
Archived |
| SEA Designator |
2011WEKUMadamMonkfishNKR |
| SEA Lysate Titer |
6.2e10 |
| Date of SEA Lysate Titering |
Oct 31, 2011 |
| Pitt Freezer Box# |
31 |
| Pitt Freezer Box Grid# |
72 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
