Mycobacterium phage Manda
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Detailed Information for Phage Manda
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByLungani Bengani
Year Found2014
Location FoundDurban, South Africa
Finding InstitutionUniversity of Kwazulu-Natal
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation TemperatureNot entered
GPS Coordinates29.81639 S, 30.94556 E Map
Discovery NotesSample CCO found outside career centre on the Westville Campus of UKZN from soil that is rarely tilled using Mycobacterium Smegmatis
Naming NotesI just thought of my girlfriend's surname! Risky but worth it!
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedOct 2, 2015
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina
Approximate Shotgun Coverage1811
Genome length (bp)76023
Character of genome ends3' Sticky Overhang
Overhang Length9 bases
Overhang SequenceCGCTTGTCA
GC Content62.8%
Fasta file available?Yes: Download fasta file
Characterization
ClusterE
Subcluster--
Cluster Life CycleTemperate
Other Cluster Members
Annotating InstitutionUniversity of Kwazulu-Natal
Annotation StatusIn GenBank
Plaque NotesBig, Round, circular, clear
MorphotypeSiphoviridae
Number of Genes147
Number of tRNAs2
Number of tmRNAs0
Has been Phamerated?Yes
Gene List

Click to View

Manda_1

Manda_2

Manda_3

Manda_4

Manda_5
(terminase small subunit)

Manda_6

Manda_7

Manda_8
(terminase large subunit)

Manda_9

Manda_10
(portal protein)

Manda_11
(capsid maturation protease)

Manda_12
(major capsid protein)

Manda_13

Manda_14

Manda_15
(head-to-tail adaptor)

Manda_16
(head-to-tail stopper)

Manda_17

Manda_18
(tail terminator)

Manda_19
(major tail protein)

Manda_20
(tail assembly chaperone)

Manda_21
(tail assembly chaperone)

Manda_22
(HNH endonuclease)

Manda_23
(tape measure protein)

Manda_24
(major tail protein)

Manda_25
(minor tail protein)

Manda_26
(major tail protein)

Manda_27
(major tail protein)

Manda_28
(major tail protein)

Manda_29

Manda_30

Manda_31
(helix-turn-helix DNA binding protein)

Manda_32

Manda_33
(lysin A)

Manda_34
(holin)

Manda_35

Manda_36
(lysin B)

Manda_37

Manda_38

Manda_39

Manda_40

Manda_41

Manda_42

Manda_43

Manda_44

Manda_45

Manda_46

Manda_47

Manda_48

Manda_49

Manda_50

Manda_51

Manda_52
(tyrosine integrase)

Manda_53

Manda_54

Manda_55
(helix-turn-helix DNA binding protein)

Manda_56

Manda_57

Manda_58

Manda_59

Manda_60

Manda_61

Manda_62

Manda_63

Manda_64

Manda_65

Manda_66

Manda_67
(exonuclease)

Manda_68

Manda_69
(ERF family ssDNA binding protein)

Manda_70

Manda_71
(HNH endonuclease)

Manda_72

Manda_73

Manda_74

Manda_75

Manda_76

Manda_77
(DNA binding protein)

Manda_78
(HNH endonuclease)

Manda_79

Manda_80

Manda_81

Manda_82
(dsDNA helicase)

Manda_83

Manda_84
(HNH endonuclease)

Manda_85
(WhiB family transcription factor)

Manda_86

Manda_87

Manda_88
(holliday junction resolvase)

Manda_89

Manda_90
(polynucleotide kinase)

Manda_91

Manda_92

Manda_93
(RNA ligase)

Manda_94
(helix-turn-helix DNA binding protein)

Manda_95

Manda_96

Manda_97

Manda_98

Manda_99

Manda_100

Manda_101
(ssDNA binding protein)

Manda_102
(ClpP-like protease)

Manda_103

Manda_104

Manda_105

Manda_106
(helix-turn-helix DNA binding protein)

Manda_107
(HNH endonuclease)

Manda_108 — tRNA
(tRNA-Arg(cct))

Manda_109 — tRNA
(tRNA-Gly(tcc))

Manda_110

Manda_111

Manda_112
(deoxycytidylate deaminase)

Manda_113

Manda_114
(DnaQ-like DNA polymerase III subunit)

Manda_115

Manda_116
(RecA-like DNA recombinase)

Manda_117

Manda_118

Manda_119

Manda_120

Manda_121

Manda_122

Manda_123

Manda_124

Manda_125

Manda_126

Manda_127

Manda_128
(DNA binding protein)

Manda_129

Manda_130

Manda_131

Manda_132

Manda_133

Manda_134

Manda_135

Manda_136

Manda_137

Manda_138

Manda_139

Manda_140

Manda_141

Manda_142

Manda_143

Manda_144

Manda_145

Manda_146

Manda_147

Manda_148

Manda_149
(HNH endonuclease)

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMK016498
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 7
Pitt Freezer Box Grid# F2
Available Files
Final DNAMaster FileDownload
GenBank File for PhameratorDownload