| Detailed Information for Phage Manda |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Lungani Bengani |
| Year Found | 2014 |
| Location Found | Durban, South Africa |
| Finding Institution | University of Kwazulu-Natal |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 29.81639 S, 30.94556 E Map |
| Discovery Notes | Sample CCO found outside career centre on the Westville Campus of UKZN from soil that is rarely tilled using Mycobacterium Smegmatis |
| Naming Notes | I just thought of my girlfriend's surname! Risky but worth it! |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Oct 2, 2015 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 1811 |
| Genome length (bp) | 76023 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 9 bases |
| Overhang Sequence | CGCTTGTCA |
| GC Content | 62.8% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | E |
| Subcluster | -- |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | University of Kwazulu-Natal |
| Annotation Status | In GenBank |
| Plaque Notes | Big, Round, circular, clear |
| Morphotype | Siphoviridae |
| Number of Genes | 147 |
| Number of tRNAs | 2 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MK016498 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
7 |
| Pitt Freezer Box Grid# |
F2 |
| Available Files |
| Final DNAMaster File | Download |
| GenBank File for Phamerator | Download |